Mouse TCR RNA Kit - Manual

The Mouse α/β TCR RNA Kit is designed for unbiased amplification of TCR alpha and beta cDNA libraries using 5’RACE (Rapid Amplification of cDNA Ends) with unique molecular identifiers (UMI) incorporated within template switch oligo. 5’RACE UMI technique allows for advanced PCR and sequencing error correction, exact quantification of template cDNA molecules, and accurate normalization of samples for comparisons of repertoire diversity metrics.