PME Free-Circulating DNA Kit-IPC16 - Manual

1 Introduction

1.1 Intended use

Free-circulating DNA in serum, plasma or in urine is very interesting as diagnostic target. The content of free-circulating DNA is usually very low and varies among different individuals. Further, the free-circulating DNA is present as short fragments, usually smaller than 1000 nt. Because of these facts, the extraction of cell-free-circulating DNA is difficult. Commercially available kits use standard nucleic acid extraction procedures based on sample lysis, binding the nucleic acids on a solid material, washing and elution of nucleic acids. Because of the high sample volume the procedures are very time and work consuming and need a lot of reagents.