SLAMseq - High-Throughput Metabolic Sequencing of RNA - Brochure

Metabolic RNA sequencing combines labeling of newly synthesized RNA transcripts with RNA-Seq readout. Existing approaches utilize nucleotide derivatives such as 4-Thiouridine and biochemical pulldown to separate nascent and existing RNA for library preparation 1. These protocols are typically cost-, time-, and labor-intensive, require high amounts of RNA input, and often produce low signal quality 1.