Ultra-Fast & Reproducible Raman Molecular Spectra Analysis for Unknown Substance Brochure

Ultra-Fast & Reproducible Raman Molecular Spectra Analysis for Unknown Substance AcuScan1500 Raman Chemical Analyzer RFD-SERS Technology Important analysis methods: • Chromatographic methods • Electrochemical methods • Optical Photometric methods Near IR, FTIR, Raman Chromatographic Methods & Electrochemical Methods Requires Sample Preparation : • organic solvent extraction • solid phase extraction • supercritical fluid extraction Optical Photometric Method has allowed the characteristic fingerprint of the substances * Assurance of Product quality and consistency rapidly * Measure multiple constituents Fingerprint simultaneously * Non-destructive testing * No sample preparation * No chemical waste, environmentally friendly * No sample waste * Analyze raw material and product on the receiving dock, in the warehouse, and QA laboratories Raman NIR Spectra Characteristics Emission Transmittance Molecules Characteristics Symmetry stretching/change in Polarizibility, size and shape Asymmetry stretching/Change in Dipole Moment Spectra Intensity Medium with SERS Low Spectra specificity high medium Water compatibility Yes No Sample Preparation No Elaborate(Eliminate water matrix) Identification Accuracy High Medium Quantitation Accuracy High High Ease of use High Medium(Need Training) Comparison of AcuScan® 1500 vs. other technologies AcuScan1500 Near IR/FTIR Chromato-graphic methods Electro-chemical methods Handheld Raman System No sample preparation required Yes No No No Yes Ultra fast detection Yes No No No Yes Sensitive Yes Yes Yes Yes Yes Automated sample identification and quantification Yes No Yes No No Reproducible Sample quantification Yes No Yes Yes No Subsance Molecules Identification Yes Yes No(UV) No No HPLC Column ON-Line Detection Yes No Yes Yes No Has RFD technology Yes No No No No Reproducibility of results Yes No Yes N/A No Peak intensity %RSD for 100 runs <0.50 N/A <1% N/A >10% PC control and software Yes Yes Yes Yes Yes Autofocus Yes No No No No Automatic position locator Yes No Yes N/A N/A Automatic Sample Drying Yes No N/A N/A N/A Universal Scan Yes No No No No Vials Yes No Yes No Yes Tablets Yes No No No Yes 96 well Yes No No No No 384 well Yes No No No No SERS plate Yes No No No Yes Customized Multi-SERS plate Yes No No No No No skilled operator needed Yes No No No Yes Easy to use Yes No No No Yes No Toxic solvents / no toxic waste Yes No No No Yes Lower operating costs Yes No No No Yes Desktop Instrument Yes Yes Yes Yes No Handheld Portable Yes No No No Yes Raman Spectra Specificity=Fingerprint of Molecules The “Raman effect” is named after its discoverer, C.V.Raman, an Indian physician. He reported different light-scattering processes araised when photons of light interact with molecules in material. Light scattering is a two-photon process, where one photon is absorbed and another photon is emitted simultaneously. When photons emitted with the same frequency as the incident photon, it is the Rayleigh scattering.(Next page, Fig. 1 ). When photons occasionally lose or gain energy due to molecular interactions and are therefore frequency shifted (Fig. 1). Raman Scattering composed of Stokes Raman scattering, whereby the photon is scattered at lower energy (shifted wavelength towards the red end of the spectrum) and anti-Stokes Raman scattering where a small number of molecules will be in a higher vibrational level, and hence the scattered photon can actually be scattered at a higher energy level (a gain in energy and a shift to higher energy and a blue shifted wavelength). Stoke Anti-Stoke Scattering Scattering Reyleigh Scattering Raman Scattering Virtual Energy Level Vibrational Energy Level Ground Energy State Incident Photon Emitting Photon Fig. 1 Industrial, Government, Enviromental Laboratory Applications: • Pharmaceuticals • Food and food contaminants/additives • Explosives • Hazardous materials • REACH • Counterfeit identification • Narcotics & intermediates or precursors • Environmental contaminants chemicals • Illicit street drugs • Chemical warfare agents • Gems and minerals • Bio-Chemicals • Clinical diagnostics (SERS) 16,000 RAMAN Substance Molecules SPECTRA DATA BASE SPECTRA # The AcuScan 1500 is AcuTech Scientific’s breakthrough... the FIRST Raman Analytical System to offer: • FAST .......... < 0.1 SECOND sample detection • SENSITIVE...Low MDQ < 0.65 PPM detection( Melamine) • HIGHLY REPRODUCIBLE RESULTS, 100 runs Signal Intensity variation %RSD < 0.5% • ROBOTIC CONTROL...automatic laser focusing for maximum signal Intensity and maximum accuracy • AUTO SAMPLE DRYING...automatic heater & vacuum drying of sample • UNIVERSAL SAMPLE TRAY...can accommodate vials, tablets, 96/384 micro-well plate, SERS plates • LOWER COST...no skilled operator nor expensive LC solvents needed • NO TOXIC WASTE...no toxic solvents to dispose • SPECTRA SPECIFICITY---molecular identification fingerprint • Automated Sample identification and quantification ---16,000 molecular-Spectral ID available • ON-Line Sample Identification and Quantification for HPLC Column Eleuents Samples: Liquid Powder Solid Sample Holders: Vials Tablets Powder plated 96 wells plates 384 wells plates SERS Data Collection: AcuScan 1500 ID Library Search: ST Japan Database or User’s In-house database AcuScan 1500 Raman Scanner System: Automated Sample Identification and Quantification Quantitation Auto-Sampler: Liquid HPLC-Column Data Collection: AcuScan 1500 ID Library Search: ST Japan Database or User’s In-house database ON-LINE-HPLC-AcuScan 1500 Raman Detection System: For Simultaneous Sample Identification and Quantification Quantitation AcuScan1500 Raman Optical Analyzer Universal Raman Scanner for Sample Holder Vials Plate Tablet Plate SERS Plate 96 well Plate 384 well Plate Customized SERS Plate Qualitative & Quantitative Reproducibility of 100 runs of polystyrene plastic Raman overlay spectra = 0.49% RSD 100 runs of polystyrene plastic, 100 spectra showed overlay in one trace 785nm 0.1 sec scan 100mW power Qualitative & Quantitative Reproducibility for 100 runs of polystyrene plastic Raman overlay spectra (0.49%RSD) Expanded view of 100 runs Raman spectra overlay at 998.36nm and 1029.57nm Raman Shifts 785nm 0.1sec scan 100mW power 100Runs of EDTA in 384 microwell plate using AcuScan1500 Plotted overlay trace to show reproducible spectra for all 100 runs Sample: EDTA powder in 384 microwell plate 0.5 sec scan time 20mW 785nm Laser power Qualitative & Quantitative Reproducibility for 100 runs of EDTA Raman Spectra Overlay 20Runs of EDTA using AcuScan1500 plotted in one overlayed trace Sample: EDTA powder in 384 microwell plate 0.5sec scan time 20mW 785nm Laser power Qualitative & Quantitative Reproducibility for 20 runs of EDTA Raman Spectra Overlay Ultra-Fast & Reproducible Raman Molecular Spectra Analysis of Sugar Aqueous Solution 100runs of 10% Betaine Aqueous solution in 96 microwell plate 785nm x 0.1 sec x 100mW Qualitative & Quantitative Reproducible for 100 runs of 10% Betaine Aqueous Solution Raman Overlay Spectra Overlay of 25 runs of 10% Fructose Aqueous solution Raman Spectra. 50mW x 0.1 sec 785nm Qualitative & Quantitative Reproducibility for 25 runs of 10% Fructose Raman Overlay Spectra Overlay of 25 runs of 10% Fructose Raman Spectra. 50mWx 0.1 sec 785nm Qualitative & Quantitative Reproducible for 25 runs of 10% Fructose Raman Overlay Spectra 25 runs of Raffinose 10% 50mW x 0.1 sec scan 785nm laser Plotted as one overlay spectra for all 25runs Qualitative & Quantitative Reproducibility for 20 runs of 10% RaffinoseRaman Overlay spectra Quantification : 1500 Application Data (Sample: 10%, 5% and 1% Betaine Solution ) 10% Betaine 5% Betaine 1% Betaine Ultra-Fast & Reproducible Raman Molecular Spectra Analysis of Cosmetics & Beauty Products Cellina Sun Plus UV Gel SPF27 SR 0.3sec scan x 100mW, Laser at 785nm Raman Analysis of Cosmetics and Beauty Product-Sunscreen Lotion Kanebo Freshel Moist Lift BB Cream 0.3 sec scan x 100 mW Laser at 785nm Raman Spectrum for Kanebo Freshel Moist Lift BB Cream NIVEA SUN Moiturising Sun Protection 30 PA++ 0.3 Sec scan x 100mW Laser at 785nm Raman Analysis of Cosmetics and Beauty Product-Sunscreen Lotion Secret Garden Tea Tree Hand & Body Lotion 0.3sec scans x 100mW 785nm Laser Raman Analysis of Cosmetics and Beauty Product-Sunscreen Lotion Ylang-Ylang Nature’s Lipcare 0.3sec scan at 25mW 785nm Raman Spectrum for Nature’s Lipcare Revlon Lipstick #725 10 runs Raman 785nm at 0.1 sec scan x 25mW Laser power Qualitative & Quantitative Reproducible for 10 runs of REVLON Lipstick Raman Overlay Spectra ESTTE LALDER #183 LIPSTICK NATURE’S LIPCARE REVLON #110 LIPSTICK 0.1sEC X3 X 100Mw 785NM Comparison of the composition of lipsticks from three companies Four most well known Handheld Raman Systems in the Market showed poor Qualitative and Quantitative Performance (10 to 20% RSD) in General. AcuScan 1500 is the only Raman Chemical Analyzer has showed 0.1 to 0.5 % RSD on spectra intensity for 100 runs of samples.------ It is the only reproducible Raman system in the market. Hillary Markert, MFS , Joan Ring, MS , Nicole Campbell, BS, Kirk Grates, BA National Forensic Science Technology Center 7881 114th Avenue North, Largo, FL 33773 (727) 549-6067 | www.nfstc.org Hillary Markert, MFS , Joan Ring, MS , Nicole Campbell, BS , Kirk Grates, BA National Forensic Science Technology Center 7881 114th Avenue North, Largo, FL 33773 (727) 549-6067 | www.nfstc.org Limited Reproducibility of Portable Raman Spectrometers Hillary Markert, MFS , Joan Ring, MS , Nicole Campbell, BS , Kirk Grates, BA National Forensic Science Technology Center 7881 114th Avenue North, Largo, FL 33773 (727) 549-6067 | www.nfstc.org Without SERS 1ppm with SERS 0.4ppm with SERS 0.1pm with SERS Conclusion AcuScan 1500 is the only Raman Chemical Analyzer has showed 0.1 to 0.5 % RSD on spectra intensity measurements for 100 runs of samples.------ It is the only reproducible Raman system for HPLC on-line and off-line simultaneous substance molecules identification and quantification in the market.