Oncology Laboratories Articles
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Review of ADC Linker and Research Progress
The linker is not only the molecule part of the covalent link between the antibody and the small molecule payload, but also a key element with design properties in targeted drug therapy. The addition of the linker should not induce aggregation and needs to ensure acceptable PK characteristics, while limiting the premature release of the payload in plasma and enabling the effective release of ...
By BOC Sciences
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Detecting Fusion Transcripts by RNA Sequencing in Screening Tumors
What is fusion gene? What is Chimeric RNAs? A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually due to chromosomal translocations or deletions. These chimeric genes can form abnormal transcripts or proteins in subsequent biological processes, which can lead to or promote the development of tumors. For example, in chronic granulocytic leukemia, ...
By CD Genomics
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Antibody Drug Conjugates: "Biological Missiles" For Targeting Cancer Therapy
Antibody-drug conjugates (ADCs) usually consist of monoclonal antibodies (mAbs) covalently linked to cytotoxic drugs via chemical linkers. With high specific targeting ability and strong killing effect, it has achieved precise and efficient removal of cancer cells, and has become one of the hot spots in the development of anticancer drugs. In 2000, the FDA first approved the ADC drug Mylotarg ...
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Three generations of ADC: Nearly a decade of development-PII
Kadcyla and second-generation non-cleavable linkers Alternative linker design methodologies are still under development. Immunogen discovered a very powerful ADC by an accidental discovery. The lysine residue of trastuzumab is linked to DM1 via a non-cleavable linker containing N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), an ADC (T-DM1, Kadcyla) authorized by the FDA in ...
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Automated Cell Segmentation and Spot Detection in Fluorescence in Situ Hybridization Staining to Assess HER2 Status in Breast Cancer
Fluorescence in situ hybridization (FISH) approach is constituted of a pair of complementary techniques for precisely detecting gene amplification and over-expression which are regarded as signs of cancer in patients. Signal detection of FISH whole slides is extremely significant as enables to detect amplification situation. However, nuclei detection and segmentation of FISH slides through ...
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Automatically Diagnosing HER2 Amplification Status for Breast Cancer Patients Using Large FISH Images
Fluorescence in situ hybridization (FISH) is a technique that prepares acceptable results for molecular imaging biomarkers to precisely and dependably detect and diagnose disorders which are sign of cancers. Since contemporary manual FISH signal analysis is low-effective and inconsistent, it is an attractive research area to develop automated FISH image scanning systems and computer-aided ...
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A Robust Computer-Aided Approach for Scoring and Quantitative Evaluation of Whole Slide Digital Images of HER2 Immunohistochemistry in Breast Cancer
HER2 is recognized as an essential prognostic parameter in breast cancer. Usually interpretation of HER2 negative cases which has no membrane staining or has faint and weak incomplete cells and also positive cases (3+) that consist of strong and complete cell are easy but cells with moderate and weak HER2 staining (2+ or equivocal) require further analysis with fluorescence in situ hybridization ...
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CD Genomics Perspective: Bioinformatic Analysis for Microbial Metagenomics
Introduction to Bioinformatics for Microbial Metagenomics In microbial ecology, metagenomic techniques are now widely utilized to research microbial communities in greater depth, including many strains that cannot be produced in the lab. Microbial bioinformatics may now be used to mine large metagenomic datasets for broad patterns that govern microbial communities. Typical metagenomic and ...
By CD Genomics
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University of Washington Medical Center - CDS-5 Cytogenetic Drying Chamber - Case Study
It’s not often you lovingly hug a 450-pound piece of equipment, but if it makes your work life easier you show your appreciation. The CDS-5 helps lab technologists create consistent and reliable environments for their work regardless of the day-to-day variables, like the weather. Imagine the weather outside impacting your office work indoors. Sounds inconvenient, right? That used to be a ...
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Quantification of target molecules needed to detect microorganisms by fluorescence in situ hybridization (fish) and catalyzed reporter deposition-fish
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is a method that is widely used to detect and quantify microorganisms in environmental samples and medical specimens by fluorescence microscopy. Difficulties with FISH arise if the rRNA content of the probe target organisms is low, causing dim fluorescence signals that are not detectable against the background ...
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