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MedChemExpress - Model ODN 1668 - ...

MedChemExpress - Model ODN 1668 - 1186063-66-0

ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’[1][2][3][4].

ODN 1668

MCE China：ODN 1668

Brand：MedChemExpress (MCE)

Cat. No.HY-150726

CAS：1186063-66-0

Storage：-20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’.

In Vitro：ODN 1668 (6-60 µg/mL; 12-72 h) increases the synthesis of S100A8/A9, IL-6 and β-galactosidase in mouse bone marrow derived macrophages[1]. ODN 1668 (24 h) promotes the activation and proliferation of B cells and increases the expression of CD86 and CD25[2]. ODN 1668 (0.3-3.0 μM; 4 days) promotes cell growth in anti-CD3-treated CD8+ T cells in a dose-dependent manner[2]. ODN 1668 (0.25-32 μg/mL; 12 h) promotes the proliferation of head kidney lymphocytes and enhances the expression of immune genes[3].

In Vivo：ODN 1668 (10 nmol ≈ 63.6 μg; injection into the hind footpads; single dose) significantly enhances antibody response in OVA treated mice and increases titers of IgG2a and IgG2b[2]. ODN 1668 (15 μg; intramuscular injection; single dose) improves the antibacterial immunity of Epinephelus laminatus infection, and has the most significant antibacterial activity in liver, spleen and kidney[3]. ODN 1668 (1-5 mg/kg; intraperitoneal/subcutaneous injection; single dose) can cause fever, anorexia and brain inflammation in rats[4].

IC50 & Target：TLR9

Sequence：DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)

References：

[1]. Rattanaprukskul K, et al. TLR9 Mediates Periodontal Aging by Fostering Senescence and Inflammaging. J Dent Res. 2022 Dec;101(13):1628-1636. [Content Brief]

[2]. Lipford GB, et al. CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants. Eur J Immunol. 1997 Sep;27(9):2340-4. [Content Brief]

[3]. Chen X, et al. CpG ODN 1668 as TLR9 agonist mediates humpback grouper (Cromileptes altivelis) antibacterial immune responses. Fish Shellfish Immunol. 2023 Jul;138:108839. [Content Brief]

[4]. Damm J, et al. Intraperitoneal and subcutaneous injections of the TLR9 agonist ODN 1668 in rats: brain inflammatory responses are related to peripheral IL-6 rather than interferons. J Neuroimmunol. 2014 Dec 15;277(1-2):105-17. [Content Brief]

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