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MedChemExpress - Model Lacto-N-neotetraose - 13007-32-4

Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure[1][2][4].

Lacto-N-neotetraose

MCE China：Lacto-N-neotetraose

Brand：MedChemExpress (MCE)

Cat. No.HY-N9445

CAS：13007-32-4

Synonyms：LNnT

Purity：99.71%

Storage：4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure.

In Vitro：Lacto-N-neotetraose (5 mg/mL, 24 h) induces IL-8 secretion in T84 cells[2]. Lacto-N-neotetraose (5 mg/mL, 24 h) reduces TNF-α induced IL-8-secretion with 38% in FHs 74 Int cells[2]. Lacto-N-neotetraose binds TNFR1 (Tumor necrosis factor receptor 1) with a Kd value of 900 ± 660 nM[2]. Lacto-N-neotetraose (5 mg/mL, 24 h) attenuates TNF-α induced inflammation by TNFR1 ectodomain shedding in FHs 74 Int cells[2].

In Vivo：Lacto-N-neotetraose (100 μM, intratracheal administration for 24 h) reduces the abundance of Streptococcus pneumonia in the lungs of pneumonia rabbits[3]. Lacto-N-neotetraose (100/200 μg, Intradermal injection, at 3, 7, 14, and 21 days post-surgery) increases the wound closure rate on day 7 post-wounding[4].

IC50 & Target：Endogenous Metabolite[1], TNFR1[2]. In Vitro Lacto-N-neotetraose (5 mg/mL, 24 h) induces IL-8 secretion in T84 cells[2]. Lacto-N-neotetraose (5 mg/mL, 24 h) reduces TNF-α induced IL-8-secretion with 38% in FHs 74 Int cells[2]. Lacto-N-neotetraose binds TNFR1 (Tumor necrosis factor receptor 1) with a Kd value of 900 ± 660 nM[2]. Lacto-N-neotetraose (5 mg/mL, 24 h) attenuates TNF-α induced inflammation by TNFR1 ectodomain shedding in FHs 74 Int cells[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Lacto-N-neotetraose Related Antibodies

References：

[1]. Xiaomin Dong, et al. CRISPRi-Guided Multiplexed Fine-Tuning of Metabolic Flux for Enhanced Lacto- N-neotetraose Production in Bacillus subtilis. J Agric Food Chem. 2020 Feb 26;68(8):2477-2484. [Content Brief]

[2]. Lianghui Cheng, et al. The Human Milk Oligosaccharides 3-FL, Lacto-N-Neotetraose, and LDFT Attenuate Tumor Necrosis Factor-α Induced Inflammation in Fetal Intestinal Epithelial Cells In Vitro through Shedding or Interacting with Tumor Necrosis Factor Rece [Content Brief]

[3]. I Idänpään-Heikkilä, et al. Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis. 1997 Sep;176(3):704-12. [Content Brief]

[4]. Behrouz Farhadihosseinabadi, et al. The in vivo effect of Lacto-N-neotetraose (LNnT) on the expression of type 2 immune response involved genes in the wound healing process. Sci Rep. 2020 Jan 22;10(1):997. [Content Brief]

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