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Pyridoxal 5'-​phosphate monohydrate

MCE China：Pyridoxal 5'-​phosphate monohydrate

Brand：MedChemExpress (MCE)

Cat. No.HY-W011727A

CAS：41468-25-1

Synonyms：Pyridoxal phosphate monohydrate

Purity：98.0%

Storage：4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Pyridoxal 5'-phosphate hydrate, the active form of vitamin B6, is an essential cofactor for multiple enzymes, including aromatic l-amino acid decarboxylase that catalyzes the final stage in the production of the neurotransmitters dopamine and serotonin. Pyridoxal 5'-phosphate hydrate is the most important coenzyme variant in the process of vitamin B6 intracellular phosphorylation and is interconvertible with other variants, including pyridoxine 5′‐phosphate (PNP) and pyridoxamine 5′‐phosphate (PMP).

In Vitro：Pyridoxal 5'-phosphate monohydrate (500 μM, 24 h) activates the ERK/c-Jun signaling pathway, promotes the expression of IGFBP1 protein in HepG2 cells[3]. Pyridoxal 5'-phosphate monohydrate (0.5 mg/mL, 48h) inhibits the proliferation of cancer cells SK-OV-3 and OVCAR-3[5].

In Vivo：Pyridoxal 5'-phosphate monohydrate (1 mg/kg, ip, single dose) alleviates Aβ25-35-induced spatial memory impairment and long-term memory impairment in mouse models[4]. Pyridoxal 5'-phosphate monohydrate (600 mg/kg/day, po for 16 weeks) inhibits the formation of advanced glycation end-products (AGEs), and prevents the progression of diabetic nephropathy in Streptozotocin (HY-13753)-induced rats diabetes model[6].

IC50 & Target：IC50: active form of vitamin B6[1] In Vitro Pyridoxal 5'-phosphate monohydrate (500 μM, 24 h) activates the ERK/c-Jun signaling pathway, promotes the expression of IGFBP1 protein in HepG2 cells[3]. Pyridoxal 5'-phosphate monohydrate (0.5 mg/mL, 48h) inhibits the proliferation of cancer cells SK-OV-3 and OVCAR-3[5]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Pyridoxal 5'-​phosphate monohydrate Related Antibodies Western Blot Analysis[3] Cell Line: HepG2

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References：

[1]. Allen GF, et al. Pyridoxal 5'-phosphate deficiency causes a loss of aromatic L-amino acid decarboxylase in patients and human neuroblastoma cells, implications for aromatic L-amino acid decarboxylase and vitamin B(6) deficiency states.J Neurochem. 2010 Jul;114(1):87-96.  [Content Brief]

[2]. Calderón-Ospina CA, et al. B Vitamins in the nervous system: Current knowledge of the biochemical modes of action and synergies of thiamine, pyridoxine, and cobalamin.CNS Neurosci Ther. 2020 Jan;26(1):5-13.  [Content Brief]

[3]. Zhang P, et al., High concentrations of pyridoxal stimulate the expression of IGFBP1 in HepG2 cells through upregulation of the ERK/c‑Jun pathway. Mol Med Rep. 2013 Oct;8(4):973-8.  [Content Brief]

[4]. Choi JM, et al., Membrane-Free Stem Cells and Pyridoxal 5'-Phosphate Synergistically Enhance Cognitive Function in Alzheimer's Disease Mouse Model. Antioxidants (Basel). 2022 Mar 21;11(3):601.  [Content Brief]

[5]. Zhang L, et al., Pyridoxine 5'-phosphate oxidase is a novel therapeutic target and regulated by the TGF-β signalling pathway in epithelial ovarian cancer. Cell Death Dis. 2017 Dec 13;8(12):3214.  [Content Brief]

[6]. Nakamura S, et al., Pyridoxal phosphate prevents progression of diabetic nephropathy. Nephrol Dial Transplant. 2007 Aug;22(8):2165-74.  [Content Brief]