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Dextran sulfate sodium salt (MW 4500-5500)

MCE China：Dextran sulfate sodium salt (MW 4500-5500)

Brand：MedChemExpress (MCE)

Cat. No.HY-116282A

CAS：9011-18-1

Synonyms：DSS (MW 4500-5500); DXS (MW 4500-5500)

Storage：Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Dextran sulfate sodium salt (MW 4500-5500) is a polymer of anhydroglucose with a molecular weight of 4500-5500. Dextran sulfate sodium salt (MW 4500-5500) can be used to induce acute colitis and cause apoptosis of colonic epithelial cells in mice. The recommended molecular weight in the study is 5000 (HY-116282) and the use concentration is 5% (in feed, W/W). The sulfated polysaccharide dextran sulfate is also an effective inhibitor of HIV. Dextran sulfate sodium salt (MW 4500-5500) can significantly inhibit HIV-1 replication at concentrations that do not significantly inhibit the blood coagulation process. Dextran sulfate sodium salt (MW 4500-5500) protects MT-4 cells from HIV-1-induced cellular pathogenicity. Dextran sulfate-induced colitis can be inhibited by Puerarin (HY-N0145), Baicalein (HY-N0196), β-Caryophyllene (HY-N1415).

In Vitro：Take Dextran sulfate sodium salt (MW 5000) as an example. Dextran sulfate sodium salt (MW 5000) (25 μg/mL; 24 h) can completely protect MT-4 cells from HIV-1-induced cytopathogenicity[1].Dextran sulfate sodium salt (DSS) was labeled with 2-aminopyridine (to obtain pyridylamino-DSS or PA-DSS) and used to detect its metabolism in Caco-2 cells. After treating Caco-2 cells with PA-DSS (MW 5000) (3%; 24 h), the cells were fixed with ethanol, permeabilized with acetone, and stained with 0.05% toluidine blue solution for 20 min. The results showed that: Dextran sulfate sodium salt (MW 5000) reacted heterochromatically in the cells, and the color development results showed that it was mainly bound to the cell nucleus and depolymerized to below MW 2500 after entering the cells[2].

In Vivo：Take Dextran sulfate sodium salt (MW 5000) as an example. Dextran sulfate sodium salt (MW 5000) induces inflammation primarily in the colon rather than in the stomach or small intestine. Dextran sulfate sodium salt (MW 5000) (3%, mixed in feed; fed for 8 days) induces colitis in mice, causing weight loss, mucosal damage, reduced cell mitotic rate, and induced apoptosis of colonic epithelial cells. Dextran sulfate sodium salt (MW 5000) is more effective than Dextran sulfate sodium salt (MW 2500) (statistically significant differences in all parameters were observed on day 6 after co-induction)[2]. Dextran sulfate sodium salt (average MW 5000) (3%, added to drinking water; gavage for 10 days) induces colitis in rats, causing blood in stool and decreased RBC and WBC indices[3]. .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } Induction of Colitis[2][3][4][5] Background Adding dextran sulfate sodium (DSS) to the drinking water of mice or rats can induce colon epithelial damage and a strong inflammatory response that lasts for several days, inducing colitis[4]. Clinical manifestations of colitis typically include watery diarrhea, hematochezia, and weight loss. The induced symptoms are very similar to human ulcerative colitis (UC) and can be used to study the occurrence and development mechanisms of acute and chronic colitis. Models can be created in many species of animals: mice, rats, zebrafish, pigs, fruit flies, etc. Specific Mmodeling Methods Mice[2]: C57Bl/6n mice • male • specific pathogen-free • 6 week-old Administration: 5% (W/W of diet) • po • 8 days Rat[3]: SPF-grade Wistar rats • male • 7 weeks oldAdministration: 3% w/v, 2 mL/kg • orally gavage (added in drinking water) • 10 days Note Dextran sulfate sodium salt (MW 5000) depolymerizes to MW 1800 molecules upon autoclaving (115°C, 15 min, 1.7 atm), with approximately 70% of the sulfate groups in each Dextran sulfate sodium salt molecule being depleted. Therefore, filter sterilization is recommended[2].

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References：

[1]. Regmi S, et, al. Enhanced viability and function of mesenchymal stromal cell spheroids is mediated via autophagy induction. Autophagy. 2021 Oct;17(10):2991-3010.  [Content Brief]

[2]. Baba M, et al. Mechanism of inhibitory effect of dextran sulfate and heparin on replication of human immunodeficiency virus in vitro. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6132-6.  [Content Brief]

[3]. Araki Y, et, al. Dextran sulfate sodium administered orally is depolymerized in the stomach and induces cell cycle arrest plus apoptosis in the colon in early mouse colitis. Oncol Rep. 2012 Nov;28(5):1597-605.  [Content Brief]