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Dextran sulfate sodium salt (MW 35000-45000)

MCE China：Dextran sulfate sodium salt (MW 35000-45000)

Brand：MedChemExpress (MCE)

Cat. No.HY-116282C

CAS：9011-18-1

Synonyms：DSS (MW 35000-45000); DXS (MW 35000-45000)

Storage：Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Dextran sulfate sodium salt (MW 35000-45000) is a polymer of anhydroglucose and is a potent inducer of colitis. Dextran sulfate sodium salt (MW 35000-45000) may be related to macrophage dysfunction, intestinal flora dysbiosis, and is particularly toxic to the colonic epithelium. Dextran sulfate sodium salt (MW 35000-45000) also inhibits human immunodeficiency virus replication by preventing viral adsorption to host cells. Dextran sulfate sodium salt (MW 35000-45000) is also used to bind to insulin, encapsulate it in gold nanoparticles, and serve as an insulin carrier to bind to insulin receptors to achieve the purpose of slowly releasing insulin and prolonging insulin activity.

In Vitro：Dextran sulfate (average molecular weight=35,000-45,000 Da, AuNPs@Dextran) can be used to target insulin through molecular recognition with a dissociation constant of approximately 5.3 μM[1].

In Vivo：Dextran sulfate sodium salt (MW 35000-45000) can be used to induce acute/chronic colitis and colitis-associated colon cancer models[6][7][8][9][10]. .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:""; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } 1. Induction of acute colitis model[6][7][8] Background DSS disrupts the intestinal mucosal barrier, activates immune cells, and triggers oxidative stress responses. These factors collectively lead to acute inflammation of the intestinal mucosa. Specific Mmodeling Methods Mice: C57BL/6 • 6-12 weeks old • 18-22 gAdministration: 1.75%-5% (w/v) DSS in drinking water • 7 days Note (1) It is recommended to replace the drinking water containing DSS every 1-2 days. The water intake can be calculated according to 7-10 mL per day for mice and 11 mL/100 g of body weight per day for rats. Ensure that there is no blockage or leakage in the water bottle. (2) Record the water intake, body weight, and disease phenotype every day. (3) The concentration of the DSS solution, the species and strain of the animals may all affect the effect of model establishment. The optimal age range for successfully and easily repeating the DSS colitis is 6 to 8 weeks, and male mice may be more sensitive than female mice. (4) In order to reduce the difference in water intake and observe the progress of model establishment more clearly, it is recommended to house 2-3 animals in each cage, and no more than 5 animals per cage. Modeling Indicators Phenotypic observation: body weight reduction within the range of 10%-20%, diarrhea, bloody stools, and a significant shortening of the colon. Histological changes (HE staining in the colon tissue): mucosal damage, crypt structure destruction, goblet cells shedding/disappearance, granulocyte infiltration. Correlated Product(s): / Opposite Product(s): P-2281 (HY-118704)；Biochanin A (HY-14595) 2. Induction of chronic colitis model[9] Background The continuous effect of DSS repeatedly damages the intestinal mucosa, leading to an imbalance in immune regulation and a dysbiosis of the intestinal microbiota. This results in the inflammation lingering and failing to heal, thus giving rise to chronic colitis. Specific Mmodeling Methods Mice: C57BL/6 • 6-12 weeks old • 18-22 gAdministration: 2%-3% (w/v) DSS in drinking water (A cycle consists of 7 days of DSS in the drinking water and 14 days with normal water, and this cycle is repeated three times.) Modeling Indicators Phenotypic observation: body weight and spleen weight reduction, diarrhea, bloody stools, rectal bleeding, and a significant shortening of the colon. Histological changes (HE staining in the colon tissue): mucosal damage, crypt structure destruction, goblet cells shedding/disappearance, inflammatory cells infiltration. Correlated Product(s): / Opposite Product(s): / 3. Induction of colitis-associated colon cancer model[9][10] Background The long-term intestinal inflammatory environment induced by DSS generates a large number of inflammatory factors and oxidative stress products, which can damage the DNA of intestinal mucosal cells, leading to gene mutations. At the same time, it disrupts the balance between cell proliferation and apoptosis, and create a microenvironment conducive to tumor growth, thus triggering colon cancer. Specific Mmodeling Methods Mice: C57BL/6 • 6-12 weeks old • 18-22 gAdministration: Intraperitoneally inject the carcinogen Azoxymethane (AOM, HY-111375) at a dose of 10 mg/kg. One week later, provide drinking water containing 2%-2.5% (w/v) of DSS (A cycle consists of 7 days with DSS in the drinking water and 14 days with normal water, and this cycle is repeated three times). Modeling Indicators Phenotypic observation: body weight reduction, diarrhea, bloody stools, loose stools, and a significant shortening and thickening of the colon. Molecular levels changes: activation of NF-κB and STAT3. Histological changes (HE staining in the colon tissue): mucosal damage, crypt structure destruction, goblet cells shedding/disappearance, inflammatory cells infiltration. Correlated Product(s): Triclosan (HY-B1119) Opposite Product(s): /

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References：

[1]. Lee KC, et al. Using Dextran-encapsulated gold nanoparticles as insulin carriers to prolong insulin activity. Nanomedicine (Lond). 2017 Aug;12(15):1823-1834.  [Content Brief]

[2]. Shen B, et al. Dextran Sulfate Sodium Salt-Induced Colitis Aggravates Gut Microbiota Dysbiosis and Liver Injury in Mice With Non-alcoholic Steatohepatitis. Front Microbiol. 2021 Nov 2;12:756299.  [Content Brief]

[3]. Li H, et al. Rabdosia serra alleviates dextran sulfate sodium salt-induced colitis in mice through anti-inflammation, regulating Th17/Treg balance, maintaining intestinal barrier integrity, and modulating gut microbiota. J Pharm Anal. 2022 Dec;12(6):824-838.  [Content Brief]

[4]. Whittem CG, et al. Murine Colitis modeling using Dextran Sulfate Sodium (DSS). J Vis Exp. 2010 Jan 19;(35):1652.  [Content Brief]

[5]. Martin JC, et al. Dextran Sulfate Sodium (DSS)-Induced Acute Colitis in the Rat. Methods Mol Biol. 2016;1371:197-203.  [Content Brief]

[6]. Kinchen J, et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018 Oct 4;175(2):372-386.e17.  [Content Brief]

[7]. Zhang H, et al. Biochanin a ameliorates DSS-induced ulcerative colitis by improving colonic barrier function and protects against the development of spontaneous colitis in the Muc2 deficient mice. Chem Biol Interact. 2024 May 25;395:111014.  [Content Brief]

[8]. Dokoshi T, et al. Dermal injury drives a skin to gut axis that disrupts the intestinal microbiome and intestinal immune homeostasis in mice. Nat Commun. 2024 Apr 8;15(1):3009.  [Content Brief]

[9]. Wirtz S, et al. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007;2(3):541-6.  [Content Brief]

[10]. Zhu H, et al. RNA virus receptor Rig-I monitors gut microbiota and inhibits colitis-associated colorectal cancer. J Exp Clin Cancer Res. 2017 Jan 5;36(1):2.  [Content Brief]