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Dextran sulfate sodium salt (MW 5000)

MCE China：Dextran sulfate sodium salt (MW 5000)

Brand：MedChemExpress (MCE)

Cat. No.HY-116282

CAS：9011-18-1

Synonyms：DSS (MW 5000); DXS (MW 5000)

Purity：99.20%

Storage：Store at room temperature, keep dry and cool In solvent -80°C 2 years -20°C 1 year

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Dextran sulfate sodium salt (MW 5000) is a polymer of anhydroglucose with a molecular weight of 5000. Dextran sulfate sodium salt (MW 5000) can be used to induce acute colitis and cause apoptosis of colonic epithelial cells in mice. The concentration dose used in the study was 5% (in feed, w/w). The sulfated polysaccharide dextran sulfate is also an effective inhibitor of HIV. Dextran sulfate sodium salt (MW 5000) can significantly inhibit HIV-1 replication at a concentration that does not significantly inhibit the blood coagulation process. Dextran sulfate sodium salt (MW 5000) protects MT-4 cells from HIV-1-induced cellular pathogenicity. Dextran sulfate-induced colitis can be inhibited by Puerarin (HY-N0145), Baicalein (HY-N0196), β-Caryophyllene (HY-N1415).

In Vitro：Dextran sulfate sodium salt (MW 5000) (25 μg/mL; 24 h) completely protected MT-4 cells from HIV-1-induced cytopathogenicity[1]. Dextran sulfate sodium salt (DSS) was labeled with 2-aminopyridine (yielding pyridylamino-DSS or PA-DSS) and used to examine its metabolism in Caco-2 cells. PA-DSS (MW 5000) (3%; 24 h) rapidly inhibited the energy metabolism of Caco-2 cells[2]. Cells were fixed with ethanol, permeabilized with acetone, and stained with 0.05% toluidine blue solution for 20 min. The results showed[2]:Dextran sulfate sodium salt (MW 5000) reacted heterochromatically in cells, and the color development results showed that it was mainly bound to the cell nucleus and depolymerized to below MW 2500 after entering the cell.

In Vivo：Dextran sulfate sodium salt (MW 5000) induces inflammation mainly in the colon rather than in the stomach or small intestine. Dextran sulfate sodium salt (MW 5000) (3%, mixed in feed; fed for 8 days) can induce colitis in mice, causing weight loss, mucosal damage, reduced cell mitotic rate and induced apoptosis of colonic epithelial cells. Dextran sulfate sodium salt (MW 5000) is more effective than Dextran sulfate sodium salt (MW 2500) (statistically significant differences in all indicators were observed on the 6th day after co-induction)[2]. Dextran sulfate sodium salt (average MW 5000) (3%, added to drinking water; gavage for 10 days) can induce colitis in rats, causing blood in stool and decreased RBC and WBC indices[3]. .f12{ font-size: 12px; } .fwb{ font-weight: bold; } .lh22{ line-height: 22px;; } .lh23 { line-height: 23px; } .pl13{ padding-left: 13px;; } .part { margin-top: 18px; } .mold-first-tit { width: 100%; height: 44px; line-height: 44px; background: #F9F7FB; border-bottom: 1px solid #EBE4F6; padding-left: 16px; box-sizing: border-box; margin-bottom: 17px; } .mold-second-tit:before { content:; width: 6px; height: 6px; display: inline-block; border-radius: 50%; background: rgba(255,102,0,0.4); margin-right: 12px; position: relative; top: -3px; } .lft-border { border-left: 1px dotted #EBE4F6; padding-right: 12px; margin-left: 3px; box-sizing: border-box; padding-bottom: 12px; } /* .part .dec:last-child { border-bottom: 0; } */ .dec { margin: 10px 15px 0; padding-bottom: 10px; border-bottom: 1px dashed #EBE4F6; } .btm-border { border-left: 1px dashed #EBE4F6; } .text-bg { margin-top: 10px; background: #FFFBF1; padding: 14px; border-bottom: 0; position: relative; } .text-note-bg { margin-top: 10px; background: #FFFDF7; padding: 12px; border-bottom: 0; position: relative; } .text-note { width: 51px; height: 20px; line-height: 20px; background: #FFE2AA; text-align: center; border-radius: 0 0 8px 0; position: absolute; top: 0; left: 0; } .text-note-dec { margin-top: 15px;; } Induction of Colitis[2][3][4][5] Background Adding dextran sulfate sodium (DSS) to the drinking water of mice or rats can induce colon epithelial damage and a strong inflammatory response that lasts for several days, inducing colitis[4]. Clinical manifestations of colitis typically include watery diarrhea, hematochezia, and weight loss. The induced symptoms are very similar to human ulcerative colitis (UC) and can be used to study the occurrence and development mechanisms of acute and chronic colitis. Models can be created in many species of animals: mice, rats, zebrafish, pigs, fruit flies, etc. Specific Mmodeling Methods Mice[2]: C57Bl/6n mice • male • specific pathogen-free • 6 week-old Administration: 5% (W/W of diet) • po • 8 days Rat[3]: SPF-grade Wistar rats • male • 7 weeks oldAdministration: 3% w/v, 2 mL/kg • orally gavage (added in drinking water) • 10 days Note Dextran sulfate sodium salt (MW 5000) depolymerizes to MW 1800 molecules upon autoclaving (115°C, 15 min, 1.7 atm), with approximately 70% of the sulfate groups in each Dextran sulfate sodium salt molecule being depleted. Therefore, filter sterilization is recommended[2].

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References：

[1]. Baba M, et al. Mechanism of inhibitory effect of dextran sulfate and heparin on replication of human immunodeficiency virus in vitro. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6132-6.  [Content Brief]

[2]. Araki Y, et, al. Dextran sulfate sodium administered orally is depolymerized in the stomach and induces cell cycle arrest plus apoptosis in the colon in early mouse colitis. Oncol Rep. 2012 Nov;28(5):1597-605.  [Content Brief]

[3]. Hori Y, et al. Effects of chondroitin sulfate on colitis induced by dextran sulfate sodium in rats. Jpn J Pharmacol. 2001 Feb;85(2):155-60.  [Content Brief]

[4]. Martin JC, et al. Dextran Sulfate Sodium (DSS)-Induced Acute Colitis in the Rat. Methods Mol Biol. 2016;1371:197-203. [Content Brief]  [Content Brief]

[5]. Jeon YD, et al. Puerarin inhibits inflammation and oxidative stress in dextran sulfate sodium-induced colitis mice model. Biomed Pharmacother. 2020 Apr;124:109847.  [Content Brief]

[6]. Zhong X, et al. Baicalein Inhibits Dextran Sulfate Sodium-induced Mouse Colitis. J Cancer Prev. 2019 Jun;24(2):129-138.  [Content Brief]

[7]. Bento AF, et al. β-Caryophyllene inhibits dextran sulfate sodium-induced colitis in mice through CB2 receptor activation and PPARγ pathway. Am J Pathol. 2011 Mar;178(3):1153-66.  [Content Brief]