ACD - Tyrosine Kinase Assays
From Primary & Transformed Cells Lines
Our tyrosine kinase assays are produced using a cell-based platform first developed by Mathey-Prevot and colleagues (learn more). The system relies on constitutively activated kinases to confer IL3-independent growth on the mouse Ba/F3 Pro B or FDC-P1 myeloid cell lines. Constitutive activation is achieved by fusing the kinase domain to donor proteins that typically (though not always) facilitate homomeric oligomerization and subsequent trans-phosphorylation. Blocking the activity of the activated kinase causes cell death, which we assess by measuring residual ATP.
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Interestingly, kinase activities and their responses to inhibitors are influenced by the activating fusion protein and the cell type in which the kinase is expressed.
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