ALiCE - Eukaryotic Expression Protein
Introducing ALiCE - a eukaryotic protein expression platform able to produce any protein at scale.
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Almost Living Cell-Free Extract
ALiCE® is a scalable eukaryotic cell-free protein expression system capable of producing even the most complex proteins in under 48 hours. Our proprietary cell-free lysate contains all of the machinery necessary to implement eukaryotic post-translational modifications, without specific optimizations.
Furthermore, the ALiCE® system consistently produces protein yields of up to an industry-leading 3 grams per liter.
Without the constraints imposed by living systems, our cell-free system has the advantage of being able to produce all proteins, even toxic ones.
ALiCE® is a plant-based cell-free lysate derived from Nicotiana. tabacum c.v. BY-2 cells. ALiCE® stands for Almost Living Cell-Free Extract. The lysate is prepared by removing the cell wall and other cell components that are not necessary for protein production, leaving only the protein production machinery and mitochondria which power the reaction. It functions just like a cell, but without the constraints of a living system.
The first scalable eukaryotic expression systemCell-free protein expression systems (CFPS) offer many benefits over cell-based alternatives, including shorter protein production time (hours vs. months), and the ability to produce proteins at levels usually toxic to living cells. However, the widespread adoption of CFPS in industrial protein manufacture has been hindered by a lack of systems that can reliably operate at scale.
In a world first, we have successfully scaled ALiCE® to produce up to 3 mg/mL protein in reaction volumes of up to 10 liters, without requiring extensive process development and optimization. This opens the door to applications of our technology beyond research and development.
To discuss ALiCE® at scale, contact our Sales Team
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