ALiCE - Protein Expression Kits
Our ALiCE cell-free protein expression kits are designed with ease-of-use in mind. Using our simple protocol will take you from reaction set-up to expressed protein in under 48 hours. All it takes is your gene-of-interest cloned into one of our vectors and the ALiCE® lysate. No additional reagents, no extra steps, and no protein-specific optimizations.
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Products Details
The ALiCE® kit offers two routes of protein expression for ultimate flexibility:
- For the expression of simple, cytosolic proteins, the pALiCE01 vector is provided. Using this vector, up to 3 mg/ml protein can be achieved.
- For expression of more complex eukaryotic proteins, delivery of your gene in the pALiCE02 vector will target your protein to the microsome (derived from Golgi and endoplasmic reticulum), for post-translational modifications.
Do you have a question about ALiCE® as a kit? Our experts are happy to answer.
ALiCE® is available as a research kit in three different sizes: mini, midi and maxi to fit your needs.
Visit our web shop to explore the options.
Our cell-free protein expression kits contain our Almost Living Cell-Free Expression (ALiCE®) technology. ALiCE® is a lysate derived from eukaryotic cells and contains all of the protein expression machinery needed to produce even the most difficult to express proteins, including those requiring post-translational modifications.
Features
- Produces up to 3 mg/ml protein in under 48 hours.
- Simple reaction set-up – just add DNA
- Capable of catalyzing complex post-translational modifications
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