GeneTex -Model CD19 -[HIB19] (PE-Cy7) -Antibody
The HIB19 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.
Application Note
- *Optimal dilutions/concentrations should be determined by the researcher.
- Application Dilution
- FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
- Not tested in other applications.
- Calculated MW
- 61 kDa. ( Note )
Synonyms
Cellular Localization
Background
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
From tissue culture supernatant
- Host Mouse
- Clonality Monoclonal
- Clone Name HIB19
- Isotype IgG1
- Application FACS
- Reactivity Human