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Aptamer Affinity Determination Technology
The determination of aptamer-target binding characteristics is an important tool in the aptamer discovery process. Affinity determination with Bio-Layer Interferometry (BLI) can be used to select aptamer candidates for specific downstream applications, identify matched aptamer pairs, and optimize buffer formulations. BLI is routinely used for characterization of aptamer candidates and identification of complementary aptamers at Base Pair. Affinity testing is available as part of an aptamer discovery project OR as a stand-alone service. Whether you have discovered aptamers with Base Pair, in your own laboratory, or with another research team, we can assist you with affinity determination.
Bio-Layer Interferometry (BLI) is an optical technique that measures changes in reflected light caused by binding at the sensor tip. White light is emitted from the sensor. It is reflected back from the sensor tip and a reference layer to a detector. A shift in the interference pattern indicates a change in thickness of the outer layer at the tip. This change is reported as a change in wavelength over time. Only analyte binding to or dissociating from the surface will cause a shift in the interference pattern. The schematic below shows changes in reflected light upon binding of aptamer and target.
- Works well in complex buffers and sample matrices
- Calculates association rate, dissociation rate, and concentration
- Can be used to identify complementary aptamers (aptamer pairs) or aptamers complementary to existing antibodies
Depending upon the number of aptamers and buffers to be tested, BLI affinity determination is typically completed within one to two weeks of receipt of labeled aptamer candidates and target. Buffers can be shipped to Base Pair or formulation(s) can be provided. Biotin-labeled or amine-labeled aptamers can be tested using standard chip formats, but testing of amine-labeled aptamers is recommended for maximum sensitivity. Custom testing formats are also available. An association constant, dissociation constant, and affinity constant (KD) are reported for each aptamer in each buffer tested.
Aptamers offer solutions to many scientific challenges other reagents can’t overcome. Learn More.
Pursue Toxic and Non-immunogenic
TargetsOvercome antibody host toxicity or failure to generate an antibody response
Target Small Molecules
Bind molecules ≥ ~60 daltons, 10-fold smaller than the smallest Ab targets
Design Stable Molecular “Switches
”Simplify assay design or modulate therapeutic function
Penetrate Tissues and Cells
Target intracellular proteins; access solid tumors
Perform Simple Chemical Modifications
Enhance affinity, stability, solubility or detection
Improve Reproducibility and Stability
Easily synthesize and re-fold aptamers
Every project is unique. Base Pair scientists work closely with each client to understand project objectives, identify technical challenges, and provide innovative aptamer-based solutions. Learn More.
Consultative Approach
We take the time to fully understand customer needs and address project challenges
Customized Solutions
Every project is carefully designed from classic and novel options for selection and optimization
Innovative Technology
Multiplex SELEX, proprietary selection algorithms and characterization techniques
Effective Aptamers
True success is defined by aptamer performance in real-world applications
At Base Pair, each phase of every project is designed to have the highest likelihood of functional success. Learn More
Phase 1: Consult
Needs Definition
Project Scope
Phase 2: Design
Library Selection
Library Modification
Target Selection
Phase 3: Select
SELEX
Next Gen Sequencing
Bioinformatics
Phase 4: Screen
High Throughput Screening
Bioinformatics
Affinity Testing
Phase 5: Apply & Evaluate
Assay Feasibility
Functional Testing
Assay Optimization
Phase 6: Mature
Truncation
Base Substitution
New Functionality