autoMACS - Model Pro - Automated Cell Isolation Separator

Full automation
Simply place samples and Reagents, choose your settings, and let the autoMACS Pro Separator perform all the automated labeling and magnetic cell separation.

Flexibility
Immediate isolation of target cells from a broad range of starting materials, which include whole blood, PBMCs and single-cell suspensions from dissociated tissues.

One column fits all
The reusable autoMACS Columns ensure high purity by positive selection, depletion, or untouched isolation of practically any target cells.

Standardization
Isolate your immune cells in a routine way each time and ensure accurate sample handling while reducing user variation and risk to operators.

• Principle: Any positive and untouched isolation can be performed to separate target cells from PBMCs, cultured cells, and dissociated tissue. In addition, cells can be directly isolated from whole blood or bone marrow using StraightFrom® Whole Blood MicroBeads.
• Overall more than 400 different MicroBeads and Isolation Kits are available and we are steadily expanding our portfolio. Upon automated separation, labeled cells as well as non-labeled cells are obtained and can be used for a variety of downstream applications such as functional and molecular assays, chimerism analysis, and flow cytometry. To ensure cell viability and integrity, cells are kept cooled in Chill Racks.
• Monitoring: Pre-set programs for all MACS® MicroBeads and Isolation Kits ensure accurate sample handling. Sensors constantly monitor the status of the autoMACS® Pro, buffer levels, and column status. This ensures optimal instrument performance and safety of samples and operators.
• Level of automation: Fully automated
• Footprint: Without MiniSampler: 605x343 mm (W x D)
• With MiniSampler: 605x455 mm (W x D)
• Height: 392.5-454 mm (adjustable touchscreen)
• It is a benchtop-sized instrument that will easily fit under a common laminar flow hood.
• Order number: 130-092-545

Characteristics: The reusable autoMACS column is specifically designed for use with the autoMACS Pro Separator delivering high cell purities and recoveries. The column is compatible with all starting materials and available MicroBeads and Isolation Kits.
One or two columns can be installed and thus increase the flexibility with regards to high recovery or high purity processes. Once installed, the columns become part of a closed fluidic system that keeps you safe when dealing with hazardous samples. Monitoring and priming of the column are automatically managed by the instrument software.
Capacity:

• Up to 4 x 109 total cells/sample
• Up to 2 x 108 magnetically labeled cells/sample
• Up to 15 mL of whole blood

Separation strategies

Positive Separation: MACS MicroBeads
StraightFrom® Whole Blood MicroBeads
MACSprep™ Chimerism and Multiple Myeloma

Untouched isolation: MACS Isolation Kits (e.g. CD4+ T Cell Isolation Kit, human; Dead Cell Removal Kit)
Indirect MicroBeads: e.g. Anti-Biotin MicroBeads

Starting material

Sample type:

• PBMCs
• Whole blood
• Bone marrow
• Single-cell suspensions from dissociated tissue or tumor
• Cultured cells (cell lines and primary cells)

Species:

• Human
• Mouse
• Rat
• Non-human primate
• any others are possible with indirect MicroBeads

Number of samples:

• 6 samples with 5 mL tubes
• samples with 15 mL tubes
• 3 samples with 50 mL tubes

Volume: 0.2 mL - 50 mL sample volume depending on MicroBeads used