Aves - Anti-Glial Fibrillary Acidic Protein Antibody (GFAP)
From Primary Antibodies
Human Glial Fibrillary Acidic Protein (GFAP) is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
Products Details
Glial Fibrillary Acidic Protein (GFAP) immunostaining (red) of mixed cortical mouse brain cultures (1:500 dilution). Secondary antibody is Texas Red goat anti-chicken IgY. The blue is DAPI staining of nuclei of all cells, including non-GFAP-positive cells. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.
- Target Glial Fibrillary Acidic Protein (GFAP)
- Format IgY Fraction
- Concentration 2 mg/mL (based on absorbance at 280 nm)
- Clonality Polyclonal
- Isotype IgY
- Applications ICC, IHC, WB
- Host Species Chicken
- Gene Name GFAP
- Molecular Weight 50 kDa
- Antigen Recombinant human GFAP expressed in Escherichia coli
- Species Reactivity Human, Mouse, Non-Human Primate, Rat
- Antibody Registry ID AB_2313547
- Storage Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
- Physical State Liquid
- Production Notes Chickens were immunized with recombinant GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
- Buffer Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ('PBS' pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
- Dilution Ranges WB: 1:1000-1:2000
- IHC: 1:500-1:1000
- ICC: 1:500-1:1000
- Quality Control Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
- Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
- Country of Origin United States
- Expiration 12 months from receipt of product
- Shipping Ice packs
- Synonyms Glial fibrillary acidic protein (GFAP)
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