Aves - Anti-NFH (Neurofilament Heavy Chain) Antibody
From Primary Antibodies
NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ['Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.
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Products Details
- Target Neurofilament Heavy Chain (NFH)
- Target Description Human Neurofilament Heavy Chain (NFH) is a 115,378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the 'neurofilament triplet' proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.
- Format IgY Fraction
- Concentration 1 mg/mL
- Clonality Polyclonal
- Isotype IgY
- Applications ICC, IHC, WB
- Host Species Chicken
- Gene Name NEFH KIAA0845 NFH
- Molecular Weight 200-220 kDa
- Antigen Native NF-H purified from bovine spinal cord
- Species Reactivity Human, Mouse, Rat
- Antibody Registry ID AB_2313552
- Storage Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
- Physical State Liquid
- Production Notes Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.
- Buffer Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ('PBS' pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
- Dilution Ranges WB: 1:5000-1:10000
- IHC: 1:2000-1:5000
- ICC: 1:2000-1:5000
- Quality Control Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
- Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
- Country of Origin United States
- Expiration 12 months from receipt of product
- Shipping Ice packs
- Synonyms Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)
- UniProt Details UniProt (Immunogen Species): P12036
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