Avioq - Model HTLV-I/II - Microelisa System
The Avioq HTLV-I/II Microelisa System is intended as a screen for donated blood and organs to prevent transmission of HTLV-I and HTLV-II to recipients of cellular blood components and as an aid in clinical diagnosis of HTLV-I or HTLV-II infection and related disease
-
Most popular related searches
Technology
Derived from purified and inactivated HTLV-I viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen, the Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of antibodies to HTLV-I/II in human serum or plasma.
Kit Configuration and Principle of Test
The product is available in three kit configurations, the 192, 576, and 9600 test kits. Please refer to the 'Literature' tab for additional information, as well as the performance characteristics of this product. The Package Insert is available in the 'Support' menu tab. The Avioq HTLV-I/II Microelisa System is an enzyme-linked Immunosorbent assay in which the solid phase (Microwells) is coated with a purified HTLV-I viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen. With the addition of a diluted test sample containing antibodies to either HTLV-I or HTLV-II, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated and the well is washed with buffer. Subsequently, anti-human immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added which binds the antibody-antigen complex during a second incubation. Following a wash and incubation with TMB (Tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution which changes the color to yellow. The amount of antibody present in the sample is proportional to color development.
Benefits
Ease of Use
- Fast, convenient procedure: Total incubation time only 2 hours, 30 minutes
- No sample pre-dilution step
Time and Cost-Savings
- Excellent sensitivity and specificity in clinical trials providing reduced cost for confirmatory testing
- High correlation between initial and repeat duplicate results
- Direct dilution into microplate well
Confidence in Results
- Excellent performance characteristics to provide the “Right Answer Sooner”
- 99.95% specificity in random donor population seen in clinical trials (95% confidence interval of 99.89 to 99.98%)
- 100 % Sensitivity as seen in clinical trials (95% confidence interval of 99.97 to 100%)
Instrument Flexibility
- 96-well microplate with 8-well strips: Adaptable to automation and your testing volume
- 20 uL specimen volume for serum/plasma
- Consistent volumes for Wash Buffer, Conjugate, Substrate, and Stop solution
Customer reviews
No reviews were found for Avioq - Model HTLV-I/II - Microelisa System. Be the first to review!