Sifin -Model TN1245 -Bacillus-Cereus-Agar (Base)
Intended use : Used for the isolation and determination of the colony count of Bacillus cereus in foodstuffs. Form : Powder. Colour : Bright green. Storage : Dry, tightly closed, at 10...25 °C. Shelf life : 3 years.
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Typical composition
- Component g/l
- Peptone 1
- Mannitol 10
- Sodium pyruvate 10
- Sodium chloride 2
- Disodium hydrogen phosphate 2.5
- Potassium dihydrogen phosphate 0.25
- Magnesium sulfate 0.05
- Bromothymol blue 0.12
- Agar 14
Directions
Suspend 39.9 g in 950 ml of distilled water and heat until completely dissolved. Autoclave at 121 °C for 15 minutes and cool down to 45-50 °C. Under aseptic conditions, add 2 bottles (= 50 ml) each of Egg Yolk Emulsion (TN 1316) and Bacillus Cereus Selective Supplement (TN 1315) to 950 ml base medium. Mix well and pour plates.
Final pH at 25 °C : 7.2 ± 0.2