WOLF -Benchtop Microfluidic Cell Sorter
The WOLF was designed and created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells of high quality effectively and easily. NanoCellect has created an entirely new type of microfluidic cell sorter that extends modern flow cytometry methods. By using proven detection technologies, NanoCellect focuses on innovative cartridge technology (commonly known as a “microfluidic chip”) and accessible, intuitive software that makes the operation simple and safe for researchers and their samples. The WOLF benchtop flow cytometry cell sorter allows for the gentle separation of cell types in bulk sorting and the isolation and dispensing of individual cells into plates. Single-cell sorting is completed in 96- or 384-well plates when using the WOLF with the N1 Single Cell Dispenser.
This flexibility and performance allows users to complete sorting experiments across different research fields and application areas like single-cell genomics, cell line development, gene editing, antibody discovery, stem cell cloning, genomic sample prep, and more.
Gentle Cell Sorting
Live cells can be damaged by conventional cell sorters, resulting in a low-quality cell population. At <2 psi, the WOLF’s microfluidic channel is gentler than any conventional cell sorter or flow cytometer, allowing for higher populations of healthy, sorted cells with improved cell viability.
Easy to use
There’s no need to be a flow cytometry expert in order to use the WOLF. The software interface is intuitive, user-friendly and simple. There is no fluidics cart, and the optics are fixed – resulting in less complexity, less clean-up, and more time for experiments.
Compact
The WOLF allows for maximum flexibility, accessibility and convenience as a benchtop cell sorter in individual labs. At 2 cubic feet, the WOLF has a small footprint and easily fits inside a biosafety cabinet.
Affordable and inexpensive to maintain
The WOLF doesn’t need a fluidics cart and uses a disposable cartridges so maintenance is simple and economical. Save money on important experiments by using an affordable and approachable cell sorter.
Sterile and disposable
The WOLF utilizes a sterile and disposable cartridge, which makes sorting biohazardous samples safe by not producing any aerosols. Cross-contamination is never an issue, as every cartridge is disposable and allows for a clean sample run, every time.
The WOLF’s performance specifications allow users to complete various types of sorts for a variety of applications that may be occurring in their lab, for the ultimate in flexibility. The WOLF can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 um, and up to 60 um. Users can expect a sorting speed of up to 300 events per second maximally. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.
Scatter Resolution: Resolves lymphocytes, monocytes, and granulocytes
Fluorescence Resolution: 8 peak separation with the Spherotech Rainbow calibration beads in the 3 fluorescence channels
Analysis Speed: >5,000 events/second
Sorting: 1 and 2 way
Back-to-back Sorting Speed: 300 events per second
Absolute Counts: Yes
Volumetric Analysis: Yes
Warm-up Time: Less than 1 minute
Sorting Output: 1.5 mL and 5.0 mL, 96- and 384-well plates
Sorting Purity: Up to 99% purity
Physical Dimensions
The WOLF is a simple, benchtop system that can easily be moved from lab-to-lab, and fits into biosafety cabinets. At 2 cubic feet and 44 pounds, researchers will not need to worry about the system taking up precious lab space.
WOLF Dimensions: 15.5W x 14.4H x 15D in (39.34 x 36.6 x 38.1 cm)
WOLF Weight: 44 lbs / 20 kg
WOLF Electrical: AC Input: 100-240V, 50-60Hz, 3-1.5A
N1 Dimensions: 8.42W x 6.5H x 8.34D in (21.4 x 16.5 x 20.9)
N1 Weight: 5.5 lbs / 2.5 kg
N1 Electrical: DC Input: 24V 1A
NanoCellect utilizes unique and proprietary microfluidic sorting cartridges for bulk sorting and single-cell dispensing. The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. Cartridges have a 100% disposable fluidic pathway, making it ideal for labs with many samples where cross-contamination or biohazard cleanup is an issue. The microfluidic cartridges are sterile and produce no aerosols, making it some of the safest sorting on the market. The cartridges also allow for maximum flexibility by allowing users to sort using their own buffers and media and only consume about 50 mL of sheath fluid in 1 day.
Sample Input: 1.5 and 5.0 mL tubes
Sheath Input: 50 mL Conical Tubes
Sheath Fluid: PBS or Buffer of Choice
Sheath Fluid Usage: 7.5 mL/Hour
Sample Flow Rate: 24 ul/Minute
Sheath Flow Rate: 160 uL/Minute
Dead Volume: 50 uL
Minimum Sample Volume: 150 uL
Tubing Diameter (Inner): 200 to 500 um
Flow Cell: 200 x 70 um
Smaller Channel Diamter: 50 um
Sample Pressure: < 2psi
Product Specs Table Optics
The WOLF uses laser-based flow cytometry technology for detecting and sorting a variety of cell types, including mammalian, plant, fungal, and bacterial cells. The WOLF utilizes typical Forward- (FSC) and Back- (BSC) scatter detectors—as well as 3 fluorescent channels excited by the blue (488 nm) laser. In combination with the N1 single cell dispenser, scientists can complete both bulk and single-cell sorting. A simple cytometer, with powerful impact, allows researchers to sort their own cells on their own bench.
Excitation: 488 nm; 30 mW diode laser; (rated at 45,000 hr life)
Laser Profile: 25 x 27 µm
Scatter Detection: Forward (0 Degrees, +/-15); Back (180 Degrees, +/-15)
Emission Detection: 3 Colors, Bandpass Filters: 525 ± 25 nm (FITC, GFP) 585 ± 20 nm (PE) 665 Longpass (PI, PE-CY5, mCherry, tdTomato)
Optical Alignment: Fixed alignment, no maintenance required
- WOLFViewer software walks users though a simple workflow by offering step-by-step wizards to make the sorting process as straightforward as possible. As a result, the software uses an intuitive workflow allowing users to quickly complete setup.
- After setup, WOLFViewer makes analyzing and creating gates simple—while still providing full-featured flow cytometry tools.
- Key tools like automatic compensation and index sorting, are streamlined and included.
- When sorting is complete, shutdown and cleanup is literally less than a minute, allowing users to rapidly conclude their work.
- The WOLF’s performance specifications support a wide variety of sorting applications across mammalian, plant, fungal and bacterial cells for cloning workflows and sample preparation work upstream of genomics analyses. The WOLF can resolve lymphocytes, monocytes, and granulocytes, as well as microbial cells down to 1 um—and larger cells up to 60 um. Users can expect a sorting speed of up to 300 events per second. Researchers have the option of 2-way sorts for bulk sorting and 1-way sorts for single-cell dispensing.
- The WOLF is a simple, benchtop system that can easily be moved from lab-to-lab or into and out of biosafety cabinets. At 2 cubic feet and 44 pounds, the WOLF provides full-featured performance without taking up valuable lab space.