Biocept - Model BRAF V600E - Target Selector Mutation Test Kit
From Target Selector Kits (US)
his kit detects BRAF V600 mutations in DNA derived from blood plasma or FFPE tissue sections to give insight into cancer characteristics and provide Biomarker status of tumors such as melanoma.
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Product Details
Benefits of Biocept Target Selector™ Switch-Blocker Technology
- Enrichment of cancer biomarker mutation BRAF V600E in a high wild-type background
- Suppression of wild-type DNA amplification
- Proofreading DNA Polymerase resulting in lower false positives
- Avoids damaged DNA templates (such as deaminated cytosines)
- Ultra-high sensitivity and specificity
- Combination with Sanger sequencing for orthogonal verification of the enriched mutation
- Detection of V600E as well as Wild-Type are quantitative
ctDNA Performance
- Sensitivity: 0.05% mutant allele fraction -single copy detection of mutant (~3pg DNA) in up to 14000 copies of wild-type DNA background
- Specificity: >99%
- Effective range of DNA input: 10pg-50ng DNA
FFPE DNA Performance
- Eliminates need for macrodissection of FFPE tissue by enriching mutations and blocking wild-type DNA amplification
- Sensitivity thresholds have been established to account for artifacts introduced by formaldehyde fixation
- Mutant allele frequency below this cut-off is considered negative for the mutation
- BRAF V600E cutoff: 0.46%
- Specificity: >99%
- Effective range of DNA input: 10pg-50ng DNA
These thresholds are provided as a guideline but every research facility/laboratory should establish their own thresholds based on their own criteria.
General Specifications
- Stability: 1 year
- Storage: 20°C
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