BIORON - RealLine Chlamydia Trachomatis
From Pathogen Detection - STIs
RealLine Chlamydia trachomatis Kits detect Chlamydia trachomatis plasmid and genomic DNA in clinical samples by Real-Time PCR. The kits are designed for in vitro diagnostics and provide qualitative detection.
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Details
Chlamydia trachomatis is an obligate intracellular human pathogen. Infections caused by C. trachomatis are among the most common sexually transmitted infections in the world, with an estimated 50 million new cases occurring worldwide each year.
The RealLine Chlamydia trachomatis Kit detects genomic and plasmid DNA. Beside the well-kown strains of Chlamydia trachomatis, this kit is detecting the Swedish variant. This RealLine Kit is also detecting the serotypes A-C, which can effect occular trachoma in the eyes.
BIORONs RealLine Chlamydia trachomatis Kits are regularly tested by external quality assessment panels, see our Quality Assurance.
Sample Material
- Scrapings of epithelial cells
- Semen
- Prostatic fluid
- Urine
Detection channels
- FAM (495 – 520 nm) for Internal Control (IC)
- ROX (575 – 602 nm) for Chlamydia trachomatis DNA
Str-format
In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted. 50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and many others.
Validated Cyclers
RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)
CFX96, iQ5 iCycler (Bio-Rad, USA)
DTprime, DTLite (DNA-Technology, Russia)
Our kits are compatible to other Real-Time Cyclers. Please read the IFU properly and ask us for recommendations of device settings!
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