TANBead -Model 621 -Blood RNA Reagent Kit
#621 is developed for Total RNA extraction of TANBead reagent family.
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- Total RNA
- Frozen Blood
- 200 ul sample volume
The fresh blood specimens are mixed with Lysis Buffer and incubated at RT for 10 minutes. Acidic phenol and BCP are applied and mixed into specimens sequentially. Next to centrifuge the specimens and collect the upper aqueous phase as samples, then mix with IPA. Final step is to usa TANBead Automated Nucliec Acid Extractors(Maelstorm series) to process and extract nueclic acid. During the process, the silicon dioxide layer coated to the magnetic beads adsorbs and purifies nucleic acids from the samples. When the program ends, collect the purified nucleic acid in a clean tube.
- Sample type
- Purified nucleic acid
- Sample amount
- Processing time
- Typical yield
- Fresh whole blood
- RNA
- 200μl fresh whole blood
- 30-40 minutes
- 2-5μg