SPACEGEN -BRCA1/2 Gene Mutations Detection Kit（Next Generation Sequencing）

The susceptible genes for breast cancer, including BRCA1 and BRCA2, are important tumor suppressor genes that play a role in homologous recombination repair (HRR) of DNA damage. Mutations in the BRCA1/2 genes can lead to homologous recombination deficiency (HRD), resulting in a significant increase in genomic instability. The variant status of the BRCA1/2 genes is of significant importance in genetic risk assessment, treatment selection, and prognosis determination for related tumors such as ovarian cancer, breast cancer, pancreatic cancer, prostate cancer, etc. The BRCA1/2 genes have relatively long sequences and diverse forms of mutations, with mutation sites scattered throughout the entire length of both genes. Therefore, BRCA1/2 gene testing must simultaneously cover the coding regions and adjacent boundary regions (ideally within ±20 bp).

BRCA and Heredity

Germline mutations in the BRCA1/2 genes originate in reproductive cells, significantly increasing the risk of developing breast cancer, ovarian cancer, and other related tumors . About 10% of breast cancer patients , 10-15% of ovarian cancer patients, and 10% of prostate cancer patientsare caused by germline mutations in the BRCA1/2 genes.

Core Technology:RingCap®

Applicable Disease:Breast cancer,Ovarian cancer,Pancreatic cancer,Prostate cancer ,Primary peritoneal carcinoma

Instruments Validated:IIllumina, Ion torrent ,MGI, etc.

Sample Type:Tumor tissue,Peripheral blood / Oral swab / Saliva

Qualification:ISO13485 certificate, CAP，CE marking

 Pack Size:16 Tests/Kit,32 Tests/Kit

1.Predict therapeutic efficacy of PARP inhibitors in patients with cancers such as ovarian cancer, breast cancer, prostate cancer ， pancreatic cancer , etc.

2.Facilitate the diagnosis of hereditary cancer syndromes, including hereditary breast-ovarian cancer syndrome (HBOC) and hereditary prostate cancer.

1.Simple Operation: Utilizing the patented RingCap® technology and pre-packaged reagents in 8-tube strips, the library preparation process is simplified, enabling shorter trun around time.

2.High Sensitivity: Detect gene mutations as low as 1% in 25ng DNA samples.

3.Comprehensive Coverage: Since there are no high-frequency mutation hotspots in the BRCA1/2 genes, sequencing of both the coding regions and adjacent boundary regions ensures comprehensive coverage in the testing process.

4.Rigorous and professional: Comprehensive and strict quality control standards, automated analysis + double verification by a professional team, truly achieving comprehensive and accurate interpretation.