AlveoliX - Breathing AXLung-on-Chip System

AX12

The AX12 is based on a 96-well plate format and consists of two chips supported by a plate. This two-part design enables the seeding of the cells directly on either side of the ultrathin membrane. Each chip includes six independent units, allowing for up to 12 samples per AX12. It is compatible with live-cell and high-resolution imaging, as well as standardized read-outs such us TEER, ELISA, rtPCR and flow cytometry.

AXDock

The AXDock connects the AX12 with the AXBreather and the AXExchanger. Two AXDock units are supplied with the system: one is installed in the incubator and the other is placed inside the cell culture hood. The AX12 is placed inside the AXDock to create an air-tight sealing, ensuring proper pneumatic function.

AXExchanger

The AXExchanger is connected to the AXDock inside the cell culture hood. It pneumatically controls the valves of the AX12, located between the inlet well, the cell culture chamber and the outlet well. By opening and closing the valves, a controlled fluid flow is created for initial chip filling and medium exchange. The AXExchanger also allows the measurement of TEER in combination with a suitable equipment. It is operated via a touch screen interface and an intuitive software.

AXBreather

The AXBreather is connected to the second AXDock placed in the incubator. It pneumatically controls the deflection of the micro-diaphragm inside the AX12, recreating the cyclic breathing motion. The breathing cycles are pre-set to 15 cycles/min (0.25 Hz) with a sinusoidal pressure curve. The AXBreather is controlled via a touch screen interface and an intuitive software. Experimental data can be exported to a USB flash drive for documentation.