CAGE Preparation Kit

CAGE Preparation Kit is a ready-to-use product that includes reagents* and the protocol necessary for the preparation of a CAGE library, which allows the easy introduction of Cap Analysis of Gene Expression, CAGE, into your R&D. * Note: Reverse Transcriptase is not inclued. CAGE enables sequencing and mapping of short tags derived from the 5`-end of mRNA, thereby quantifying the frequency of tag sequences. A distinctive feature of CAGE is its capability to accurately identify promoter sites for each transcript and obtain gene expression profiles based on identified promoters. A sequencing run using one channel on the Illumina Sequencer can yield over 4,000,000 reads per sample. Expression profiles obtained from DeepCAGE (i.e., combination of next-generation sequencing with next-generation expressing profiling) will be powerful tools for various gene expression analyses and genome annotation projects.