- Home
- Companies
- Cambio Ltd
- Products
- Cambio - Agarose Gel-Digesting Enzyme
Cambio - Agarose Gel-Digesting Enzyme
Cambio Agarose Gel-Digesting Enzyme is a unique enzyme solution developed for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. This product is an agarase derived from a heat-resistant microorganism found in the deep oceans. It has excellent heat resistance compared to conventional agarase.
Agarose Gel-Digesting Enzyme digests the carbohydrate backbone of molten agarose into small, soluble oligosaccharides (neoagaro-oligosaccharides). While the agarose solution after the degradation does not form a gel again. This property allows Agarase to be used for extracting nucleic acid from agarose gels.
The advantages of nucleic acid extraction by Agarose Gel Digesting Enzyme include the following:
- Simple Protocol
- Non -hazardous reagents
- Low melting point agarose compatible
- Relatively large DNA fragments can be recovered without damage.
- Suitable for gels made with TAE, TBE, MOPS, or phosphate buffers
- Extremely stable at -20°C, 4°C and ambient for long periods
- Retains 100% activity after >120 Freeze thaw cycle
Composition
Thermostable β-Agarase (30 units / 300 units)× 1 Vial
Activity
1 unit/ μl
Storage Conditions
50 mM NaCl, 20 mM Tris-HCl (pH 7.5)
Definition of Units
One unit is defined as an enzyme activity that produces reducing
sugar equivalent amount of 1 μmol D-galactose from agarose gel per
1 min at 60°C.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200