CellSieve - Microfiltration for Circulating Tumor Cells
Personalized Cancer Therapy and Disease Monitoring: “Liquid biopsy” using circulating tumor cells (CTCs) is potentially a minimally invasive alternative to traditional tissue biopsy to determine cancer therapy. Enumeration of CTCs may be used to indicate prognosis and to monitor treatment. Isolation of CTCs by size exclusion is a widely researched technique to collect CTCs from patient peripheral blood. Creatv produces precision CellSieveTM microfilters with dense, uniform pores. Microfiltration using CellSieveTM takes only minutes and provides high capture efficiency.
Specifications
- Biocompatible polymer
- Pore diameter: 7 µm
- Filter size: 13 mm
- 160,000 pores within 9 mm diamter
Comparison with Track Etch Microfilters
Size Selection
- CTCs are larger & less plastic than blood cells
- Precise pore size and distribution deliver consistently higher capture efficiency and lower background contamination
Capture Efficiency Study
- Comparison with track etch filters
- MCF-7 cells spiked into 7.5 ml of whole blood
- Filtered by negative pressure in < 2 min
- Stained with Acridine Orange
CellSieve Microfiltration Properties
Microfilter Properties and Features
- Not fluorescent
- Lies flat on microscope slide
- Transparent
- Biocompatible
- High tensile strength (75 Mpa)
- Uniform pore size, high porosity
- Straight, parallel pores
- Thermally stable to 150°C
- Acid, alkali and solvent resistant
Capture Efficiency Study
- 5 cell lines
- Cells spiked into 7.5 ml whole blood
- Filtered by negative pressure in < 2 min
CellSieve Microfiltration Capabilities
CellSieve Benefits
- High CTC recovery
- Reproducible results
- Rapid sample processing
- High quality imaging of cells
- Not dependent on surface marker expression for capture
CellSieve Applications
- Enumeration
- Fluorescence in situ hybridization (FISH)
- Surface marker assays
- Nucleic acid assays
- Culture
- Enzymatic activity
- Histological staining
- Sequencing
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