Claret - Primer Set Kit
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Each base kit type is offered in 24- and 96-reaction format. The CBS-UD- primer sets (teal) allow Unique Dual Indexing, but they are not compatible with the UMI addition option. For UMI addition choose the UM- primer sets (red). Choosing the CBS-UM- primer sets also provide the primer and reagents for UMI addition, and primers for dual indexing PCR. If UMI addition is not required, the user may use their own Illumina® compatible index PCR primers by choosing “None”.
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Refer to the “KIT CONTENTS NITTTY GRITTY” section below for more information about each module and subsequent sections for more information for UMI and UDI considerations
Please refer to Appendixes A, B in the unabridged manual for more details on Index PCR primer set-up and UMI-aware sequencing set-up and beads purification strategies, respectively. Contact Illumina® technical support for more information about sequencer specific set-up for UMI-aware sequencing.
Please refer to this application note for comparison metrics between the Clarefy™ beads and other commercially available DNA purification beads. Please refer to Appendix C for specialized DNA purification protocols to retain short fragments.
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