Claret - Srsly Technology for DNA
From Our Technology
SRSLY - our Single Reaction Single-stranded DNA Library Prep Kit. SRSLY is a simple and efficient ligation-based single-stranded DNA library preparation method that is engineered to produce complex libraries from samples with limited inputs. SRSLY recovers complete double-stranded DNA molecules, even when they contain nicks, plus single-stranded DNA molecules inaccessible to most NGS prep kits. Furthermore, the freedom of choice during DNA cleanup allows the user flexibility to decide whether they want to retain or discourage a substantial portion of short fragments. Did we mention it does all of this, from sample to sequencer, in under 3 hours without altering the native ends of cell-free DNA molecules? Seriously.
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Product Details
No matter your sample type, we got you - biologically degraded nucleic acid samples (cfDNA, forensic samples, ancient DNA), DNA sheared in vitro, synthetic oligonucleotides or probes, damage DNA (FFPE), and even first-strand cDNA.
SRSLY is versatile. It works well with a variety of inputs due to its unique molecular and informatic features. First off SRSLY renders all input molecules single-stranded before ligating novel splint adapters. This feature enables the direct sequencing of synthetic oligos and first strand cDNA products without the need for duplex hybridization or second strand synthesis. This feature also recovers template molecules containing DNA nicks and labile lesions, such as those that often afflict FFPE and ancient DNA (aDNA) samples. Secondly, SRSLY is a directional preparation method that retains both the native 5’ and 3’ ends of each template molecule by foregoing end-polishing. This feature improves fragmentomic analysis of cell-free DNA (cfDNA), where non-random DNA fragmentation reflects critical biological information. Finally, SRSLY is fine-tuned to capture a substantial fraction of short DNA fragments. Consequently, the method is a powerful tool for difficult input types such as forensic and aDNA samples as well as for cfDNA researchers seeking to enrich for DNA derived from DNA binding proteins, mitochondrial and microbial DNA.
SRSLY works in a one-step combined phosphorylation...
SRSLY works in a one-step combined phosphorylation/ligation step that simultaneously prepares template DNA molecules for ligation without end-polishing and simultaneously ligates proprietary Illumina-compatible adapters.
Specifications
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From DNA to sequence-ready Illumina® library in 2.5 hours
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Options available for single and dual-indexing, as well as unique molecular identifier (UMI) incorporation
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Single reaction and minimal reagents reduces failures due to error, reduces bench time
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Additional protocols to retain fragments < 100 bp
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