Puracyp - Culture and Dosing Media
Our culturing and dosing media are formulated to produce optimal activation. Each cell line requires its own culturing and dosing media that is specially formulated. Kits include these media preparations but, extra media is available. Our cryopreserved cells also require Puracyp`s specialized media preparations for optimal results.
Catalog Number: Volume (ml)
- DPX2-C50-100: 50
- DPX2 C125-100: 125
- 1A2-DRE C50-101: 50
- 1A2-DRE C125-101: 125
- RPXR C50-102: 50
- RPXR C125-102: 125
- DPX2 D50-100: 50
- DPX2 D125-100: 125
- 1A2-DRE D50-101: 50
- 1A2-DRE D125-101: 125
- RPXR D50-102: 50
- RPXR D125-102: 125
Employing reporter genes governed by the P450 promoters allows for accurate gene activation from the ligand-activated receptor. Correlations between binding affinity and activation are more readily predicted.
When ligands bind to the full-length receptor they can produce conformational alterations that influence the extent of gene transcription. Our competitors employ only the ligand binding domain (LBD) of the receptor.
Low variability and high reproducibility with results comparable to those obtained in primary cultures.,Simple and easy to perform, with completion in 24 to 48 hours.,Indirect NR activators can be identified. Indirect activators cannot be detected with mammalian two-hybrid or ligand binding assays.,Puracyp is the only nuclear receptor (NR) company that has functional assays allowing agonists to be differentiated from antagonist. Our competitors assays only determine binding to the LBD. These assays cannot identify agonism vs antagonism.