Dolomite - Model nadAROSE - Reagent Kit
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Construct the future of single cell research with the nadAROSE kit. A simple, low cost, commercial solution for the encapsulation of up to a million single cells in individual micro-scaffolds in a high throughput, reproducible manner.
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Products Details
Why use agarose for your single cell projects?
Agarose’s spherical scaffold structure and ability to allow for the diffusion of nutrients and gases make it an ideal system for naturalistic mammalian and non-mammalian live cell studies, given its many similarities to the native physiological environment of living cells. Single cells encapsulated in agarose can, therefore, be maintained for extended periods of time within these individual three-dimensional microenvironments and used in various research including single cell experiments.
Applications
nadAROSE downstream applications
Control over monoclonality for your downstream workflows, with applications in:
- Cell secretion studies
- Cell-cell interactions
- Microbial cell culture
- Compatible with cytometric analyses
Terminology
We recognize that scientific terminology can be vague or confusing and we want to let you know exactly what our technology does!
The nadAROSE kit produces small spheres of hydrogels which contain cells. We call these cell-containing Scaffolds. They provide a structure to contain the cells and their products or a matrix through which they can propagate.
We differentiate scaffolds from spheres of aggregated or cultivated cells which are often termed Spheroids. Likewise, Organoids is term used to describe a collection of cells with recognisable organ characteristics.
- Scaffold – small sphere of hydrogel that contains a cell(s)
- Spheroid – spherical aggregation of cells (sometimes can mimic characteristics of tissue)
- Organoid – a simple organ, produce in vitro, which demonstrates some recognisable organ characteristics.
Features
- High throughput encapsulation of up to 1 million cell in one run
- No microfluidic knowledge required – all scaffold production done with single use, prefabricated consumables
- Throughput flexibility choose to run 1, 2, 4 or 8 lanes at once
- Very high sample compatibility – broad cell size range
- Compatible with cytometric analyses – cell-containing agarose scaffolds can be flow-processed for cell analysis
- Experimental flexibility – cell-containing agarose scaffolds are suitable for a range of experimental procedures
- Temperature controlled process for robust encapsulation and scaffold construction
- Compatible with cell release
- The nadAROSE kit produces agarose scaffolds that can be later digested to release cells
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