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- Model CCPA96 - ELISA Kits
Model CCPA96 - ELISA Kits
Enzyme immunoassay for the detection of specific RA antibodies (anti-CCP2) of IgA class in human serum or plasma. Microtitre wells are coated with the citrulinated synthetic peptides of 2. generation as an antigen. If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete). The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
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Advantages:
- The total assay time is about 2 hours.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (10, 20, 130, 800 IU/ml).
- Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in IU/ml.
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
- Detection of high specific anti-CCP2 antibodies allows early diagnosis of Rheumatoid arthritis (RA).
- Checking of therapy results and determination of disease prognosis using the quantitative determination.
Brief assay procedure:
- Dilute samples (1:101).
- Pipette Controls and diluted Samples.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Conjugate.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×
- Pipette Substrate (TMB-Complete).
- Incubate at 37°C for 30 min.
- Pipette Stop Solution.
- Read color intensity at 450 nm.
- Evaluate the test.