Genemed - Model SA-20003-1AH - Hamster (Armenian) Serum IgG, Purified
From Secondary Antibodies & Elisa Kits - Isotype Control (Ig`s)
A healthy non-immunized population of Hamster (Armenian) was used to collect serum. IgG was prepared by ammonium sulfate, and column chromatography (Sephadex, and DEAE-ion exchange columns). The resulting preparation was judged to be >95% pure by IEP and SDS-Page.
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Product Details
Form and Storage
The normal Armenian hamster IgG is provided as liquid (1 mg/ml in PBS pH 7.4) or in lyophilized form. The product should be stored at 4 oC for short term and –20oC for long term storage. It is stable for a minimum of 1 year. Do not store diluted solutions. Sodium azide, an inhibitor of peroxidase, must be avoided if used with antibody-HRP conjugates.
The lyophilized products should be dissolved in PBS, pH 7.4 to prepare desired concentration by gentle rocking or vortexing at room temperature. Centrifuge the IgG solution briefly (10,000 g, 15 min) to remove any particulate matter. It should be aliquoted and stored frozen for long term use.
Suggested Uses
This preparation of normal Hamster IgG is suitable for coating the ELISA plates or as a non-immune control for ELISA, dot blot, Western or IHC. Recommended testing concn. is approx. 1-10 ug/ml. It is, however, possible that this preparation may be unsuitable for some antigens or cell/tissue extracts and may give slightly higher background. Switching to another preparation from different mouse or vendor may alleviate this problem.
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