Model AL5 -Genomics Software
Cross species/ taxa-specific assays, Species identification assays
- Gene splicing/alternative splicing using microarrays
- Exon junction primer design
- Real-time PCR primer design
- TaqMan® probe design
- SYBR® Green primer design
- Molecular beacon design
- FRET probe design
- Microarray probe design
- NEAT assay design
- Database support
- Input & output formats
New features in the latest version of AlleleID (7.81)
- Now compatible with Mac OS X Mavericks (10.9).
- Accommodates the latest genomic databases at the NCBI
AlleleID® is a comprehensive desktop tool designed to address the challenges of bacterial identification, pathogen detection or species identification. With ClustalW multiple sequence alignment at its core, AlleleID® can be used to design species identification/cross species probes for microarrays or real time PCR including SYBR® Green, TaqMan® MGB, TaqMan® probes, Molecular Beacons and real time PCR primers. AlleleID® also offers support for designing microarray experiments for detecting alternative splicing events.
AlleleID is the only software product that supports real time PCR primer design and microarray probe design. Applications include bacterial identification, cross species research, species specific analysis, strain identification, allele specific oligonucleotide design, gene splicing detection, alternative splicing detection and splice variant analysis
Species Identification Assays/Cross Species Assays/Allele identification Assays
AlleleID® aligns sequences using ClustalW and analyzes conserved and species specific regions. You can then use the program for real time PCR primer design (SYBR® Green primer design included) and dual labeled probe design (TaqMan® probes, TaqMan® MGB probes and molecular beacons). These assays are designed to detect only the strain (strain detection) or species of interest from the mix.
For cross species assays, AlleleID® identifies the conserved regions to design a universal probe. For related organisms, AlleleID® can be used to study gene expression when genome draft of the organism under study is not available. This powerful functionality is sure be helpful for many challenging tasks such as detection, identification, quantification or monitoring of contaminants, environments, etc.