Model H3A4 (4H5) -Hepatitis B Core Antigen Antibody
Mouse anti Hepatitis B Core Antigen antibody, clone H3A4 (4H5) specifically recognizes Hepatitis B virus core antigen (HBcAg), the highly immunogenic 21kDa replicative form of the Hepatitis B virus (HBV), expressed by infected cells. Antibodies to HBcAg are produced by the host during and following an acute HBV infection and can be detected in chronic HBV carriers and patients recovered from HBV infection, usually persisting in the body for life.
- Target Species: Viral
- Product Form: Purified IgG - liquid
- Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution: Phosphate buffered saline
- Preservative Stabilisers: 0.01% Thiomersal; 50% HRP Stabiliser (BUF052A)
- Immunogen: Recombinant Hepatitis B core antigen
- Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Regulatory
For research purposes only
Applications of Hepatitis B Core Antigen antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/100,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
HRP-conjugated clone H3A4 may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with clone H6F5 as the capture antibody.