ReverTra Ace -High Efficient Reverse Transcriptase
ReverTra Ace is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.
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Features
- RNase minus M-MLV RTase with improved performance
- Enables the synthesis of longer cDNAs (≥ 14 kb) than the WT-enzyme
- Exhibits excellent reaction efficiency at high temperatures.
Applications
- cDNA synthesis
- cDNA library construction
- RT-PCR
- 5’ RACE
Source
- E. coli strain carrying a cloned, modified M-MLV reverse transcriptase gene.
Unit definition
- One unit is defined as the amount of enzyme required to incorporate 1 nmole of dTTP into an acid-insoluble material in 10 min at 42ºC.
Storage condition
- 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 10 mM DTT, 0.01% Nonidet® P-40, 50% Glycerol. Store at -20ºC
Components
- ReverTra Ace® (100U/μl)
- 5 x Buffer