RayBio - Model C5 - Human Cytokine - Membrane-Based Antibody Array

Antigen Information
Gene Symbols:ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20| CCL22 | CCL23 | CCL24 | CCL26 | CCL4 | CCL5 | CCL7 | CCL8 | CSF1 | CSF2 | CSF3 | CX3CL1 | CXCL1 | CXCL10 | CXCL12 | CXCL13 | CXCL2 | CXCL3 | CXCL5 | CXCL6 | CXCL8 | CXCL9 | EGF | FGF4 | FGF6 | FGF7 | FGF9 | FLT3LG | GDNF | HGF | IFNG | IGF1 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IL10 | IL12A | IL13 | IL15 | IL16 | IL1A | IL1B | IL2 | IL3 | IL4 | IL5 | IL6 | IL7 | KITLG | LEP | LIF | LTA | MIF | NTF3 | NTF4 | OSM | PDGFB | PGF | PPBP | SPP1 | TGFB1 | TGFB2 | TGFB3 | THPO | TIMP1 | TIMP2 | TNF | TNFRSF11B | TNFSF14 | TPO | VEGFA

Assay Format
Number of Targets Detected:80
Species Detected:
Human
Compatible Sample Types:
Cell Culture SupernatantsCell LysatesPlasmaSerumTissue Lysates
Design Principle:
Sandwich-based
Method of Detection:Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:Membrane

Product Specifications
Size:2, 4, or 8 Sample Kit

Introduction
The cytokine array is an antibody based immunoassay similar to ELISA, but able to simultaneously measure multiple proteins on one solid support. The Cytokine Array C5 is a membrane-based antibody array, using a panel of 80 different capture antibodies printed on a nitrocellulose membrane. The protein from the incubated sample is captured, and a detection antibody is also bound, creating the antibody-antigen-antibody sandwich. C-Series arrays are processed similarly to a western blot and the entire procedure can be completed in 1 day with little to no optimization.

Product Features

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Species Detected
Human

Application Notes
Suggested Application: Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components

• Human Cytokine Antibody Array C5 Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Biotinylated Detection Antibody Cocktail
• Streptavidin-Conjugated HRP
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates
• Manual

Other Materials Required

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVP`s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE`s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

1. Block membranes
2. Incubate with Sample
3. Incubate with Biotinylated Detection Antibody Cocktail
4. Incubate with HRP-Conjugated Streptavidin
5. Incubate with Detection Buffers
6. Image with chemiluminescent imaging system
7. Perform densitometry and analysis

Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.