Human IFN-alpha 2 Quantikine HS ELISA Kit

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The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.

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Measurement: Quantitative ELISA

Detection Method: Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate: HRP

Reactivity: Human

Specificity: Natural and recombinant human IFN-alpha 2.

Cross-reactivity: < 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference: No significant interference observed with available related molecules.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to asses inter-assay precision. Assays were performed by at least three technicians

Assay Length: 4.0 hours

Sample Type & Volume Required Per Well: Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Sensitivity: 0.107 pg/mL

Assay Range: 0.781 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)