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Creative Enzymes - Model DIA-413 - Hydroxysteroid Dehydrogenase
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In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD(P)+ ↔ 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.
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- Abbr 3α-HSD, Recombinant (B. choshinensis)
- Source B. choshinensis
- Species B. choshinensis
- Enzyme Commission Number EC 1.1.1.50
- Bio-activity > 30 U/mg lyophilizate
- Appearance White lyophilizate
- Molecular Mass ca. 41 kDa
- pH Stability 6.0–10.0
- Michaelis Constant 2.4 x 10^-5 M (androsterone) 3.0 x 10^-6 M (NAD)
- Structure 2 subunits of 25 kDa (SDS-PAGE)
- Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADH per min at 25°C and pH 8.9.
- Optimum pH 11
- Optimum temperature 50–60°C
- Stabilizers Trehalose
- Thermal stability below 45°C
- Stability stable at 37°C for at least four weeks
- Synonyms hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD(P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50