BioMag -Model Magnetic and Amine -Immobilization Kit
BioMag® Amine consists of a suspension of magnetic iron oxide particles coated to provide primary amine groups. The amine groups allow for the covalent attachment of proteins or ligands with retention of biological activity. BioMag® Amine may be purchased separately or as a component in the BioMag Magnetic Immobilization Kit, which is designed to introduce the user to the ease of magnetizing biological molecules. The BioMag® Magnetic Immobilization Kit includes BioMag® Amine, glutaraldehyde (the reagent used to couple proteins to BioMag®), a BioMag® Separator, and a Reaction Flask. Supplied with the kit is 10mL of BioMag® suspension, sufficient to attach up to 100mg of most proteins. A coupling efficiency of greater than 80% is achieved for many proteins. Higher amounts of protein can be attached at lower coupling efficiencies.
Proteins can be covalently attached to BioMag® Amine by any of the reagents used to prepare affinity supports provided the solid phase terminates with a primary amine group.1 A suggested glutaraldehyde procedure is given below which follows the method of Weston and Avrameas.