Epithelix -Model MUCILAIR -In Vitro 3d Human Upper Airway Epithelium
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MucilAir is an in vitro cell model of the human airway epithelium cultured at the air liquid interface.
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Since 2006 we produce MucilAir™ with high standards of quality and reproducibility
- Reconstituted using human primary cells at low passage (P1)
- Remains fully differentiated and functional for over one year in culture
- Ready and easy to use
- Cultured in chemically defined serum-free medium
- Available worldwide in 24 well format
MUCILAIR™ IS AVAILABLE FROM SINGLE DONOR OR POOL OF DONORS
- Single donor or pool of donors available in nasal healthy version
- Different anatomical sites (Nasal, Tracheal or Bronchial)
- Patients with several pathologies:
- Healthy
- COPD (Chronic Obstructive Pulmonary Disease)
- Asthma
- Cystic Fibrosis (several mutations available)
- Allergic Rhinitis
- Smoker
MUCILAIR™ CHARACTERISTICS
- Air-liquid interface
- Production of mucus
- Active cilia-beating
- Active ion transport
- Tight junctions
- Metabolic activity
- Cytokines, chemokines, metalloproteinases release
MORPHOLOGY AND HISTOLOGY OF MUCILAIR™
- Radial cut of MucilAir™: C=Ciliated cells; M=Mucus cells; B=Basal cells.
- Cross section of cilia, the microtubules’ organization is visible.
- Tight junction between two cells of MucilAir™.
- Beginning of ciliogenesis.
CILIOGENESIS OF MUCILAIR™
A time course of cilia formation monitored by anti-β tubulin immunostaining over 60 days.
After 45 days the epithelia are fully differentiated and ciliogenesis is complete, the result can be observed by Electron Microscopy:
VIDEOMICROSCOPY OF MUCILAIR™
Videomicroscopy of a MucilAir™ epithelium reconstituted from a Healthy donor. Microbeads were applied on top of the epithelium, we can see an organization of the mucociliary clearance.