Lena Biosciences - Model POP - Perfused Organ Panel
From Perfused Organ Panel
The POP microphysiological system is a lab technology that combines the 3D cell culturing capabilities of our SeedEZ scaffold with the advanced oxygenation and pH stability of our Blood Substitute within a compact, perfused multi-well insert plate, PerfusionPal. The system is ideally suited for modeling interstitial perfusion that cells in most tissues experience in vivo, and the majority of tumors bigger than 2 mm. With our proprietary Blood Substitute that mimics the functions of hemoglobin, pericellular perfusion carries sufficient amount of respiratory gases to enable long-term cell survival and improved cell functions in vitro.
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Our Lab is going beyond Organ-on-a-chip to create Perfused Organ Panel, a microphysiological system with a Blood Substitute that allows users to model multiple organs in one biomimetic platform.
What is Perfusion?
Perfusion describes the rate at which blood is supplied to a given organ in the body. It is a crucial factor in cell culture methods that positively impacts cell survival mechanisms in vitro as well as simulates real life growth conditions for your research.
Perfusion delivers oxygen to regulate metabolic activity of cells and promotes dissipation of heat. Poor perfusion may lead to cell death, and on a larger scale tissue necrosis. Our technology is tunable to deliver specific perfusion rate needed for your model.
In vivo, hemoglobin contained in the red blood cells delivers over 98% of oxygen necessary for tissue homeostasis, energy production and drug metabolism. Our Perfused Organ Panel is the first microphysiological system that provides blood-like perfusion and oxygen delivery to tissues cultured in vitro via our hemoglobin analog, Blood Substitute.
How it works:
- Key in perfusion rate and place PerfusionPal plate in the incubator.
- Media is changed and tested for metabolites or cytokines as in any multiwell plate.
- The risk of contamination is negligible. The only liquid in the tube is the Blood substitute in which microorganisms do not grow.
- There is no cross-talk between wells/ cultures. Each culture is perfused in its own medium that floats atop the Blood Substitute. Blood substitute is twice as dense as medium, immiscible with and insoluble in media, reagents and drugs.
- Once studies are complete, culture media is removed with a pipette and discarded. The Blood Substitute that remains in the tray is collected, sterile filtered, and reused for subsequent studies.
Benefits
- Physiologically relevant interstitial perfusion
- Superior oxygenation and pH stability via Blood Substitute that mimics the functions of hemoglobin
- High cell sensitivity to stimuli
- Statistically independent wells (high N for tests and controls)
- Long-term culturing
- In-well assaying (supernatants can be transferred to another plate for reads)
- Assay multiplexing
- Time-course assaying by sampling concentrated secretome and metabolome from wells
Features
- Sterile and ready to use
- Customizable perfusion rates
- Aseptic perfusion (Blood Substitute does not support growth of microorganisms)
- Reliable perfusion (no bubbles and no microchannels to clog)
- Zero dead volume in fluidics (no waste of precious reagents)
- Blood Substitute can be reused, and maintains purity
- Blood Substitute is inert, insoluble in and immiscible with media, reagents, drugs etc.
- Better precision and control compared to animal models
Applications
PerfusionPal's innovative technology concentrates the secretome, metabolome and signaling molecules produced by the cells in the well. These molecules may be concentrated enough to facilitate detection of new biomarkers of efficacy or therapy surveillance.
Applications include:
- Small molecule drug testing: drug metabolism, on-target efficacy & off-target toxicity
- Large molecule biopharmaceutical testing: interstitial delivery mimicking in vivo conditions, on-target efficacy/ off-target toxicity of antibody-drug-conjugates & multi-specific antibodies
- Cancer research: simulate advanced, vascularized tumors with heterogeneous cell populations, extracellular matrix, & intra-tumor supply of drugs to more closely mimic a patient’s tumor at the time of treatment
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