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​CSR Biotech
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  5. CSR Biotech - Model MI-SIM - Machine ...

CSR Biotech - Model MI-SIM - Machine Intelligent Structured Illumination Microscope

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MI-SIM represents a comprehensive super-resolution microscopy solution for live cell imaging, entirely conceived, developed, produced, and marketed by CSR Biotech. This advanced technology was born from the collaborative efforts of an interdisciplinary team at Peking University, renowned for their groundbreaking work in biomedical imaging. As a tool of choice, MI-SIM offers exceptional capabilities for observing live cell dynamics with ultra-high spatial and temporal resolution.

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cell imaging
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With its exceptional spatial resolution surpassing 60 nm, swift imaging capabilities reaching up to 1500 frames per second, and an expansive field of view (FOV) of 150 μm with a 100 × objective, the MI-SIM sets new standards in 3D super-resolution imaging of various dynamic processes in living cells. Notably, MI-SIM's advanced sensitivity and reduced photo-toxicity facilitate prolonged imaging sessions while minimizing photobleaching effects.

MI-SIM boasts a suite of sophisticated features, including real-time super-resolution, multi-camera and multi-site imaging capabilities, and intelligent region-of-interest (ROI) planning. It also excels in stitching within large FOV, 3D real-time tracking, multimodal focusing, and event-driven tracking. These capabilities underscore MI-SIM's versatility, ease of use, and user-centric design, reflecting CSR Biotech's leadership in the realms of advanced imaging, online decision-making, and intelligent control systems.

2D/3D Realtime Super Resolution

First to realize real-time SR imaging, seeing is obtaining
Previewing at an 85 nm resolution, our system delivers a clear visualization of mitochondrial inner membrane structures, surpassing the resolution of standard preview methods. It boasts real-time reconstruction speeds of 24 Hz, with the capability to reach up to 65 Hz, facilitating the rapid acquisition of targeted samples for imaging. By bringing super-resolution (SR) imaging into the digital era, we significantly enhance imaging efficiency. This progress allows for quick evaluation of image quality and facilitates the prompt adjustment of regions of interest (ROI) and imaging protocols.

60 nm Super Resolution

First to achieve 60 nm resolution in live-cell imaging with extremely low photobleaching Marking a pioneering advancement, our system is the first peer-reviewed technology to attain 60 nm resolution at the living cell level through a blend of optical and computational innovations. It facilitates detailed visualization of every marker and its distribution within living cells, enabling the observation of dynamic structures like the nuclear pore protein NUP98's ring structure, which also has a size of 60 nm.

Long-term Live-cell Tracking

World's 1st to realize real-time intelligent tracking for long-term live cell imaging Enhanced Tracking Precision: TRACKER revolutionizes long-term live cell imaging with its advanced ability to automatically adjust for 3D defocus and 2D shifts, ensuring clear focus and tracking accuracy despite cellular motion and morphological changes. Comprehensive Multi-ROl Observation: Designed for detailed analysis, TRACKER adeptly monitors multiple regions of interest (ROIs), capturing crucial morphological and quantitative changes in cell growth, phagocytosis, and division for in-depth, long-term cellular studies.
  • Imaging Modes
    • All 25 modes:
    • Widefield: brightfield, fluorescent widefield, oblique (multi-angle)
    • TIRF: TIRF (multi-angle), TIRF-SIM (multi-angle)
    • 2D SIM: 2D-SIM (multi-angle), 2D-SIM Stack (multi-angle)
    • 3D SIM: 3D-SIM (multi-angle), 3D-SIM Slice (multi-angle)
  • Resolution
    • XY：60 nm †
    • Z：200 nm †
  • Imaging Speed
    • Max. 49 fps @16 bit/2048 × 2048 (with sCMOS ORCA-Fusion BT)
    • Max. 564 fps @16 bit/144 × 2048 (with sCMOS ORCA-Fusion BT)
    • Max. 330 fps @8 bit/2048 × 2048 (with sCMOS Kinetix)
    • Max. 699 fps @8 bit/1024 × 3320 (with sCMOS Kinetix)
    • Max. 1500 fps @8 bit/206 × 2048 (with sCMOS Kinetix)
  • Field of View
    • Max. 150 × 150 μm @100 × magnification
    • Max. 250 × 250 μm @60 × magnification
  • Lasers
    • Compatible multi-color coupled lasers
    • Standard: 405 nm、488 nm、561 nm、640 nm
    • Optional: 445 nm、473 nm、515 nm、532 nm、594 nm、607 nm、647 nm ☆
  • Objectives
    • Suitable for 20 ~ 100 × with immersion of oil, silicone oil, water and air
  • Multi-channel Imaging
    • High-speed sequence imaging with multi-color wheel
    • Multi-FOV, multi-channel with multi-camera imaging
  • Post-processing
    • Computational SR, deep learning, denoise, background removal, co-localization analysis, etc.