Mcherry - Model 0020 - Anti-mCherry Chicken Primary Antibody
From Primary Antibodies
Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, dsRED, RFP (all tested) and tdTOMATO (by similarity) and is purified. It is provided in liquid form.
Products Details
Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
- Target mCherry
- Target Description mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.
- Format Affinity purified
- Concentration 0.2 mg/mL
- Clonality Polyclonal
- Isotype IgY
- Applications ICC, WB
- Host Species Chicken
- Gene Name MCHERRY
- Molecular Weight 27 KDa for native monomer
- Antigen Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli
- Antibody Registry ID AB_2910557
- Storage Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
- Physical State Liquid
- Production Notes Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
- Buffer Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,' pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
- Dilution Ranges WB: 1:500-1:1000
- ICC: 1:100-1:500
- Quality Control Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.
- Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
- Country of Origin United States
- Shipping Ice packs
- UniProt Details UniProt (Immunogen Species): X5DSL3
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