Nexcelom - Model Cellaca MX - High-throughput Automated Cell Counter
From High-throughput Automated Cell Counters
The High-Throughput Automated Cell Counter that Changes Everything, Your workflow will be streamlined and more efficient when you integrate the Cellaca MX with these capabilities:
Features
- Batch samples – run up to 24 samples at a time
- Small sample volume – only 25 µl of cell sample required
- Autofocus – fast autofocus prior to analysis
- Cell based assays – data output at a single cell level enables you to do cell-based assays such as apoptosis, mitochondrial membrane potential, protein expression (including GFP and RFP), and reactive oxygen species (ROS)
- Time course reporting – effortlessly create growth curves with multiple samples at several time points using the customization tools
- Automatic calculation – calculate various data points such as doubling time for individually labeled samples with an easy-to-use interface
- Multiple configurations – choose to add up to five fluorescent filters
- Automation ready – robotic integration ability with optional API
- Customizable reports – add graphs, images, charts, and tables
- Multi-language support – over 7,000 languages available
- 21 CFR Part 11 ready – optional add-on that includes audit trail, user access control, and digital signature
Run 24 samples in less than 3 minutes! No more reloading of individual slides or waiting for small batch counters to finish counting. With the Cellaca MX High-throughput Automated Cell Counter your experiments are no longer limited by the number of samples you can quickly and accurately count.
Multiple fluorescent filter options enable you to perform additional cell-based assays in minutes. With the Cell Fitness Panel you can get a quick check of the following cell health parameters:
- Viability
- Vitality
- Apoptosis
- Reactive oxygen species (ROS)
With the Cellaca MX you can build your own assays or choose from the many assays already included in the software. In addition, there are over 200 cell types preloaded with optimized settings. Simply find your assay and cell type in the menu and you are ready to count.
As soon as the first well has been acquired you can begin reviewing the images and data. No need to wait until the entire batch has been analyzed before starting to assess the results. Answers are available in seconds, not minutes.
The ability to add additional automation with robotic integration can further increase productivity.
Number of Samples |
Amount of Time | |
Cellaca MX | 24 480 |
48 seconds 30 minutes |
Cell Counter V | 12 480 |
36 minutes 24 hours |
The Cellaca MX has undergone rigorous testing and validation to ensure highly accurate results. In comparisons to manual counting with a hemocytometer, and Cellometer automated counters the Cellaca MX proves to be a highly accurate instrument. In addition, the Cellaca MX can warn the operator when the cell count is out of range (too few or too many cells in the sample) and will suggest a corrective course of action (concentrating or diluting the cell sample and by how much).
In this study, three tubes of 5-micron polystyrene beads were diluted to a concentration of ~2×106 beads/mL. To determine the concentration within each tube, 40 individual hemocytometer counts were performed for each tube. The Cellaca MX was then used to measure the concentration on the same set of tubes. The results show a very close correlation between the hemocytometer counted beads and the Cellaca MX counts.
Nexcelom Counting Plates are made in the USA to exacting standards. This ensures accurate cell counts you can trust across manufacturing lots of Nexcelom Counting Plates.
In a study of 1,992 individually loaded samples (83 plates) by multiple users over multiple weeks a count of ~ 2×106 cells/mL CHO-s cells with trypan blue yielded a CV less than 6% (figure 3). The cumulative counting time for all 83 plates was 70 minutes.
Count messy samples more accurately with fluorescence! With the Cellaca MX 2FL and 5FL models, you can eliminate red blood cells, platelets and debris from your counts using fluorescent nuclear stains. Acridine orange and propidium iodide are perfect for counting the nucleated cells in samples like PBMCs, patent samples, tumor digests and samples with lots of debris.
The advantage of fluorescent counting for primary cellsThe images (Figure 4) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.
Count Clumpy Cells using Declustering AlgorithmWhen working with clumpy cells you can apply declustering settings to identify and individually count each cell in your sample to get highly accurate counts.
Export reports as CSV, Excel, Word, or PDF files. Use default reports or customize based on the data and images you want included for the assay that was run. Statistical analysis can be included for various parameters. Different languages are available for reporting.
Nexcelom’s unique proprietary patented technology allows the user to mix the cell sample and the viability dye or staining reagent in a single well within the plate. The built-in capillary mechanism ensures even distribution across the wells. Therefore, providing a completely processed sample set for imaging, accurate counting, and analysis. There is no longer the need to perform these steps in a separate tube.
An optional module can be purchased to meet the FDA requirements for 21 CFR Part 11. The additional module comes with the following features:
- User login with passwords
- User assigned permissions
- Audit trail
- Error log files
- Electronic signatures
There are many parameters that can indicate the fitness of your samples but this is the first time that these parameters have been combined into a single kit to allow you to better examine samples of interest. These individual tests provide potential insight into sample quality. These tests can be run on the Cellaca MX FL2 or FL5. The Cell Fitness Panel includes the following tests:
Viability – AO/PI (Acridine Orange and Propidium Iodide) – Using AO/PI allows for the identification, enumeration of mononuclear cells, and provides cell viability in the counted sample without a RBC lysing step.
Vitality/Enzymatic Activity – Calcein AM/PI – By looking at the conversion of non-fluorescent calcein AM to green fluorescent calcein we can measure the number and concentration of both metabolically active live cells as well as non-viable cells in a given population.
Reactive Oxygen Species – Total ROS – While reactive oxygen species (ROS) are natural by-products of normal metabolism, during oxidative stress-related events, the accumulation of ROS can result in significant damage to cell structures. Cultures that are positive for ROS may signal unhealthy culture conditions that can lead to apoptosis and cell death.
Mid-Stage Apoptosis – Annexin V/PI – An assay commonly carried out by researchers interested in performing functional assays to measure the percent of live, apoptotic, and necrotic cells in a sample. May serve as an early indicator of an unhealthy cell population.
Late-Stage Apoptosis – Caspase 3/7 – Early or mid-stage apoptotic cells may recover or may progress to late stage apoptosis. A cell culture with a high number of caspase 3/7 positive cells may be in trouble. In this assay the executioner caspase is detected as a bright nuclear signal.
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