Nexcelom - Model Cellometer Auto 2000 - Cell Viability Counter
From Fluorescent Automated Cell Counters
The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas.
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Features
- Pipette 20 µl
- Insert Slide
- Select Assay & Click Count
- View Results in 30 seconds!
The Auto 2000 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
- Increase throughput
- Increase accuracy
- Improve consistency
- Ensure all data is correctly captured
- Count difficult cells (clumpy, irregular-shaped)
- Eliminate judgment errors, miscounts, interference from red blood cells and user-to-user variability
The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:
- Nucleated Cells for Transplantation
- PBMCs for Immunology
- Splenocytes for Vaccine Development
- Stem Cells for Cellular Therapy
- Tumor Cell Suspensions for Oncology
Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both ‘messy’ and ‘clean’ samples enables the Auto 2000 to evaluate samples at a variety of points throughout sample processing – from initial collection to separation, to cryopreservation.
The Cellometer Auto 2000 features one-touch assays for analysis of a wide range of primary samples, including:
The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.
The advantage of fluorescent counting for primary cellsThese images (right) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells, and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.
Analysis of Clumpy and Irregular-shaped CellsNCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes.
- 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size
- All 59 NCI-60 cell lines have been successfully validated on the Cellometer Cell Counter
All 40 of the NCI Comprehensive Cancer Centers use Cellometer Cell Counters.
Clumpy CellsThe MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown above).
The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.
The user-friendly touch screen displays pre-optimized assays for common cell types. User-specific protocols can be easily created and saved to the menu. The simple, pre-optimized assays make the instrument easy to operate and simplify training for new users.
With the click of a button, researchers can select a new assay with saved counting parameters, making the instrument ideal for labs testing a variety of cell types. Auto-save options allow users to quickly test a series of samples.
Because bright field cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The Auto 2000 is equipped with standard assays for dual-fluorescence analysis of primary cells stained with AO/PI.
The AO/PI MethodAcridine Orange, AO, is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium iodide, PI, can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.
With the Auto 2000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.
Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Bright field counted images can be viewed for basic cell counting and trypan blue viability.
Fluorescent counted images indicating counted live and dead nucleated cells can be viewed for dual-fluorescence primary cell viability assays.
Users can confirm that:
- cells are counted correctly, based on size and shape
- cells within clumps are being counted individually
- red blood cells, platelets, and debris are being excluded from results
The bright field image confirms that individual cells within pairs are being counted and smaller debris is not being counted. In the combined fluorescent counted image, live counted cells are circled in green. Dead counted cells are circled in red.
- Cell images can be archived and exported for use in publications and presentations.
- Saved images can be re-counted using default or user-optimized analysis settings
The Auto 2000 Cell Counter Automatically generates a cell size histogram based on cell diameter.
Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time
The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.
This example demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.
Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 2000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds.
- Eliminate Wash Steps
- Eliminate Judgment Errors
- Eliminate interference from RBCs
- Eliminate Recording & Calculation Errors
- Reduce Counting Time … Run More Experiments
Time savings – no washing
No risk of cross-contamination
Reduced biohazard risk to users
Controlled sample volume
Large-depth chambers for large cells
Most affordable automated counting consumables
The Auto 2000 allows users to generate specific folders to which data reports and images can be auto-saved. Using the auto-save feature ensures all data is accurately captured in the correct location. It also enables users to test a series of samples very quickly
Users can configure the Auto 2000 to request a new sample name for each sample that is imaged and counted or apply auto-numbering to individual samples in a series to further reduce analysis time. Users can also apply a time stamp to individual data files.
Experienced Nexcelom Technical Support Specialists are available from 8:30am to 5:00pm EST for phone and on-line support and can assist with:
- Creation of new cell types
- Optimization of counting parameters
- Troubleshooting
- Training of new users
- Installation of the Auto 2000 Cell Viability Counter
The help button at the bottom right of the Cellometer Auto 2000 software screen gives users instant access to:
- Software features and instructions
- On-line tutorials and training videos
- Submission of a Support Ticket
All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image Cytometry.
Nexcelom Field-based Applications Specialists are also available for:- On-site demonstrations
- Training
- Troubleshooting
- Technical Seminars
Applications
Automatically measure cell size of freshly isolated adipocytes and plot size histogram. DNA staining fluorescence dyes are used to identify cells from lipid droplets. »
Adoptive Cell Transfer TherapyUse Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »
Cell Viability Measurement Using Trypan Blue or AO/PIWhen should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »
Cell Viability and NecrosisCell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »
TNC Concentration & Viability for Clinical (Blood) SamplesAnalyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »
Cell Size AssayPerforming cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »
NCI-60 Cancer Cell LinesAutomatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »
Rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection automatically. »
Immunology ResearchAutomatically quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, slpenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »
Insect CellsAutomatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »
Peripheral Blood Mononuclear Cells (PBMC)Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »
PlateletsAutomatically measure mouse and human platelet concentration using brightfield only method. »
Stem CellsAutomatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »
WBCs in Whole BloodAutomatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »
Specifications
Includes
- Cellometer Auto 2000 Instrument with integrated computer and touch-screen and dual-fluorescent optics
- Cellometer Software
- Two USB 2.0 ports
- Power Supply
- Ethernet port
- Phone/online applications support during set-up
Imaging Performance
Cell Size: 5 – 300* microns
Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications
Weight: 26 lbs. (11.8 kg)
Dimensions: Width: 11.1″ (28.3 cm)
Depth: 12.8″ (32.4 cm)
Height: 13.4″ (34.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.5A
Output to Instrument: 12 VDC, 7.5A
Display
10.4″ touch screen
Fluorescent Optics
Excitation / Emission: 470 nm/535 nm
Example Fluorophores:
- Acridine Orange (+DNA)
- Calcein AM
- CFDA
- SYTO® 9
- SYTO® 13Excitation / Emission: 540 nm/605 nm
Example Fluorophores:
- Propidium Iodide
- Ethidium Bromide
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