Nexcelom - Model Cellometer Auto 2000 - Cell Viability Counter

1. Pipette 20 µl
2. Insert Slide
3. Select Assay & Click Count
4. View Results in 30 seconds!

• Increase throughput
• Increase accuracy
• Improve consistency
• Ensure all data is correctly captured
• Count difficult cells (clumpy, irregular-shaped)
• Eliminate judgment errors, miscounts, interference from red blood cells and user-to-user variability

The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

• Nucleated Cells for Transplantation
• PBMCs for Immunology
• Splenocytes for Vaccine Development
• Stem Cells for Cellular Therapy
• Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both ‘messy’ and ‘clean’ samples enables the Auto 2000 to evaluate samples at a variety of points throughout sample processing – from initial collection to separation, to cryopreservation.

The Cellometer Auto 2000 features one-touch assays for analysis of a wide range of primary samples, including:

The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.

These images (right) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells, and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes.

All 40 of the NCI Comprehensive Cancer Centers use Cellometer Cell Counters.

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown above).

The user-friendly touch screen displays pre-optimized assays for common cell types. User-specific protocols can be easily created and saved to the menu. The simple, pre-optimized assays make the instrument easy to operate and simplify training for new users.

With the click of a button, researchers can select a new assay with saved counting parameters, making the instrument ideal for labs testing a variety of cell types. Auto-save options allow users to quickly test a series of samples.

Because bright field cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The Auto 2000 is equipped with standard assays for dual-fluorescence analysis of primary cells stained with AO/PI.

Acridine Orange, AO, is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium iodide, PI, can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

With the Auto 2000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Bright field counted images can be viewed for basic cell counting and trypan blue viability.

Fluorescent counted images indicating counted live and dead nucleated cells can be viewed for dual-fluorescence primary cell viability assays.

Users can confirm that:

• cells are counted correctly, based on size and shape
• cells within clumps are being counted individually
• red blood cells, platelets, and debris are being excluded from results

• Cell images can be archived and exported for use in publications and presentations.
• Saved images can be re-counted using default or user-optimized analysis settings

Counting 1 x 10⁶ cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 2000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds.

Experienced Nexcelom Technical Support Specialists are available from 8:30am to 5:00pm EST for phone and on-line support and can assist with:

• Creation of new cell types
• Optimization of counting parameters
• Troubleshooting
• Training of new users
• Installation of the Auto 2000 Cell Viability Counter

All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image Cytometry.

• On-site demonstrations
• Training
• Troubleshooting
• Technical Seminars

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When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

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Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »

Automatically measure mouse and human platelet concentration using brightfield only method. »

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

Automatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »