Nexcelom - Model Cellometer Spectrum - Image Cytometry System
From Fluorescent Automated Cell Counters
The Spectrum Image Cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned templates. Quickly plot cell population data as a histogram, scatter plot, dot plot or contour plot using FCS express. The filter sets are user-changable, allowing you to customize the instrument for specific assays/reagents of interest.
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Features
Basic cell counting, primary cell viability, and cell-based assays.
Dual-Fluorescence for Accurate Primary Cell ViabilityNo interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.
User Changeable Fluorescence FiltersChoose from six color options to run two-color assays
Unique Algorithms for Advanced Cell AnalysisDetermine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.
Fast ResultsObtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.
- Small 20 µl sample size
- Cell count, concentration and viability
- Two-color antibody assays
- GFP/RFP Transfection
- Cell health and cell-based assays, including:
- Apoptosis
- Cell Proliferation
- Cell Cycle
- Mitochondrial Potential
- Phagocytosis
- Surface Marker Analysis
View Cell Images
- Visually check cell morphology
- Ensure only cells of interest are counted
- Archive and re-analyze cell images
- Export images for publication
Proprietary Pattern-Recognition Software
- Count individual cells in clusters
- Count irregular-shaped cells
- Count cells based on size
- Eliminate debris from cell counts
Non-Fluidic Platform
- Disposable counting chambers – no washing
- Compatible with fragile cells
- Maintenance-free
- Robust optics modules and LED light sources
IQ/OQ Validation and GMP/GLP Accessories
- Installation Qualification reagents/protocol
- Operational Qualification reagents/protocol
- On-site IQ or OQ Performance
- GMP/GLP Software Module
- Automatic identification of Acridine Orange (AO) stained cells (shown above)
- Image and count up to 2×107 cells/mL
- Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
- Versatile platform – use for routine cell counting and complex cell-based assays
- Determine cell viability, for cell lines or primary samples, using AO/PI in seconds
- Accurately measure cell samples with varying viability (0 – 100%)
- Export image data into flow cytometry software: FCS express
- Simple workflow: No fluid- stream, No PMT voltages, No forward/side scatter
- Easily perform data analysis using pre-designed templates
- Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot
User-Changeable Fluorescence Optics Modules*
The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.
Cell Types for Many Research Areas
Optimized for primary cell analysis
PBMCs
Stem Cells
Adipocytes
Neural Cells
Hepatocytes
Dendritic Cells
Epithelial Cells
Keratinocytes
Lymphocytes
Splenocytes
Monocytes
- Clinical Immunology: PBMCs
- Diabetes / Obesity: Adipocytes
- Immunotherapy: Leukocytes
- Microbiology: Yeast (Spectrum 10x)
- Oncology: Cell Lines
- Regenerative Medicine: Stem Cells
- Toxicology: Hepatocytes
- Transplantation: Nucleated Cells
- Vaccine Development: Splenocytes
Cellometer Spectrum Reagents
- ViaStain™ AO Staining Solution (CS1-0108-5mL)
- ViaStain™ PI Staining Solution (CS1-0109-5mL)
- ViaStain™ AOPI Staining Solution (CS2-0106-5mL, CS2-0106-25mL)
- ViaStain™ Calcein-AM/PI Cell Vitality/Viability Kit (CSK-0118)
- ViaStain™ Calcein AM ( CS1-0119)
- DAPI (CS1-0127-2mL)
- Hoechst 33342 (CS1-0128-5mL)
- ViaStain™ Hoechst/PI Viability Kit (CSK-V0005-1)
- ViaStain™ CFDA-AM Yeast Vitality Stain (CSK-0125-200µl)
- ViaStain™ Yeast Kit for Live/Dead Concentration (CSK-0102-2mL, CSK-0102-10mL)
- Yeast Dilution Buffer (CS0-0110-500mL)
- Annexin V-FITC (CS1-0114)
- ViaStain™ PI Staining Solution (CS1-0116)
- Annexin V Binding Buffer (CS0-0115-100ML)
- CaspGLOW™Fluorescein Active Caspase-3 Staining Kit (K183-100-N, K183-25-N)
- CaspGLOW™Fluorescein Active Caspase-8 Staining Kit (K188-100-N, K188-25-N)
- ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture (CS1-V0002-1)
- ViaStain™ Live Caspase 3/7 Detection with Hoechst (CS1-V0003-1)
- ViaStain™ Cellometer PI Cell Cycle Kit (CSK-0112)
- ViaStain™ Total Cell Nuclear Green (CSK-V0008-1)
- ViaStain™ Total Cell Nuclear Red (CSK-V0009-1)
- ViaStain™ Total Cell Nuclear Far Red (CSK-V0010-1)
- ViaStain™ Dead Cell Nuclear Green (CSK-V0012-1)
- ViaStain™ Dead Cell Nuclear Red (CSK-V0013-1)
- ViaStain™ Dead Cell Nuclear Far Red (CSK-V0014-1)
- ViaStain™ Dead Cell Nuclear Blue (CSK-V0015-1)
- ViaStain™ CMFDA (CS1-P0001-1)
- ViaStain™ CFSE (CS1-P0002-1 )
- ViaStain™ Cell Fitness Panel for Cellometer Spectrum (CSK-V0023-1)
Specifications
Includes
- Cellometer Spectrum Instrument
- Cellometer Software
- FCS Express 6 analysis software (2 licenses)
- Two Fluorescent Optics Modules
- Power Supply
- USB 2.0 Connection Cable
- Phone/online applications support during set-up
Magnification Selection
- 5x (ex: most mammalian cells)
- 10x (ex: yeast, platelets, algae)
Imaging Performance
5x magnification
Cell Size: 5 – 300* microns, Conc. Range: 105 – 107 cells/ml10x magnification
Cell Size: 0.5 – 25 microns, Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.* Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications
- Weight: 24.0 lbs. (10.9 kg)
- Dimensions: Width: 6.0” (15.2 cm), Depth: 8.5” (21.6 cm), Height: 14.0” (35.6 cm)
- Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
- Output to Instrument: 12 VDC, 3.34A
PC / Laptop
Minimum
Requirements(If purchasing Cellometer without PC laptop)
- Windows 10
- 2 GHz or higher processor
- 4GB RAM (8GB RAM or higher recommended)
- USB 2.0 or higher
- Display resolution: 1024 x 768 pixels or higher
Intel Mac with Parallels/Running Windows 10
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